Ovate family protein1 interaction with BLH3 regulates transition timing from vegetative to reproductive phase in Arabidopsis
Ovate family protein1 interaction with BLH3 regulates transition timing from vegetative to reproductive phase in Arabidopsis
复制标题
卵状家族蛋白1与BLH3的相互作用调节拟南芥从营养期到生殖期的转变时间
DOI：
10.1016/j.bbrc.2016.01.135
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发表时间：
2016
影响因子：
3.1
通讯作者：
Chang Ying
中科院分区：
文献类型：
--
作者：
Zhang Liguo;Zhang Xiaofei;Ju Hanxun;Chen Jingui;Wang Shucai;Wang Hemeng;Zhao Yuanling;Chang Ying
三氨基酸环延伸（TALE）同源结构域转录因子BLH 3调节从营养期到生殖期的过渡时间。之前使用大规模酵母双杂交获得的初步结果表明，BLH 3蛋白可能与Ovate家族蛋白（OFP）转录辅助调节因子相互作用。OFP 1-BLH 3复合体是否参与拟南芥营养生长期向生殖生长期转变的调控尚不清楚，本研究利用酵母双杂交系统对BLH 3与OFP 1的相互作用进行了验证。我们发现BLH 3-OFP 1的相互作用主要是通过BLH 3同源结构域介导的。同时，这种相互作用进一步证实了在体内的双分子荧光互补（BiFC）。此外，通过建立原生质体瞬时表达，我们发现BLH 3作为一个转录激活因子，而OFP 1作为一个阻遏物。OFP 1与BLH 3之间的相互作用可降低BLH 3的转录活性。p1突变系和blh 3突变系、OFP 1过表达系和BLH 3过表达系均能影响水稻从营养生长期向生殖生长期过渡的时间。此外，35 s：OFP 1/blh 3植株的开花和叶片数量与野生型对照相似。35 s：BLH 3/ofp 1植株开花早，叶片数量少于野生型对照，表明OFP 1蛋白可能部分依赖于BLH 3来调节从营养期到生殖期的过渡时间。这些结果支持了我们的假设，即通过与OFP 1相互作用，BLH 3形成了一个功能性蛋白复合物，控制从营养阶段到生殖阶段的进展时间，OFP 1可能负调控BLH 3或BLH-KNOX复合物，这是维持从营养阶段到生殖阶段的正常过渡的重要相互作用。
Three-Amino-acid-Loop-Extension(TALE) homeodomain transcription factor BLH3 regulates timing of transition from vegetative to reproductive phase. Previous preliminary results obtained using large-scale yeast two-hybrids indicate that BLH3 protein possibly interact with Ovate Family Proteins(OFPs) transcription co-regulators. Nevertheless, it is uncertain whether OFP1–BLH3 complex is involved in regulation of timing of transition from vegetative to reproductive phase in Arabidopsis.The interaction between BLH3 and OFP1 was re-tested and verified by a yeast two-hybrid system. We found that the BLH3–OFP1 interaction was mainly mediated through the BLH3 homeodomain. Meanwhile, this interaction was further confirmed by bimolecular fluorescence complementation (BiFC)in vivo. Further, by establishing protoplast transient expression, we discovered that BLH3 acts as a transcriptional activator, whereas OFP1 functioned as a repressor. The interactions between OFP1 and BLH3 can reduce BLH3 transcriptional activity. Theofp1mutant lines andblh3mutant lines,OFP1overexpress lines andBLH3overexpress lines can both influence timing of transition from vegetative to reproductive phase. Furthermore,35s:OFP1/blh3plants exhibited flowering and leaf quantity similar to that of the wild-type controls.35s:BLH3/ofp1plants flowered earlier and had less leaves than wild-type controls, indicating that OFP1 protein might depend partially on BLH3 in its function to regulate the timing of transition from vegetative to reproductive phase. These results support our assumption that, by interacting with OFP1, BLH3 forms a functional protein complex that controls timing of progression from vegetative to reproductive phase, and OFP1 might negatively regulate BLH3 or the BLH-KNOX complex, an important interaction for sustaining the normal transition from vegetative to reproductive phase.