In vivo monitoring of circadian timing in freely moving mice
In vivo monitoring of circadian timing in freely moving mice
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DOI：
10.1016/j.cub.2008.02.024
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发表时间：
2008-03-11
期刊：
影响因子：
9.2
通讯作者：
Takumi, Toru
中科院分区：
文献类型：
--
作者：
Nakamura, Wataru;Yamazaki, Shin;Takumi, Toru
在哺乳动物中，驱动日常生理和行为节律的主要昼夜节律起搏器位于下丘脑前部的视交叉上核（SCN）[1]。SCN的神经输出对于行为活动的昼夜节律调节至关重要[2，3]。虽然在揭示SCN内昼夜节律产生的分子基础方面已经取得了显着进展[4-6]，但SCN对昼夜节律施加控制的输出途径尚未得到很好的理解。大多数SCN传出神经靶向室旁下区（SPZ），其位于SCN的背侧[7，8]。这种输出途径被认为是昼夜节律调节流出的主要组成部分[9，10]。我们通过自由活动小鼠的多单位神经活动（MUA）[11]检查了中枢时钟的下游通路。SCN神经活动与环境光输入紧密耦合，并与SPZ的MUA节律密切相关。在表现出减弱的昼夜运动节律性的Clock突变小鼠中[12-14]，SCN和SPZ中的MUA节律性类似地减弱。这些结果表明，SPZ在将昼夜节律和光信号传递到参与产生行为活动的中心中起着功能性作用。
In mammals, the principal circadian pacemaker driving daily physiology and behavioral rhythms is located in the suprachiasmatic nucleus (SCN) in the anterior hypothalamus [1]. The neural output of SCN is essential for the circadian regulation of behavioral activity [2,3]. Although remarkable progress has been made in revealing the molecular basis of circadian rhythm generation within the SCN [4-6], the output pathways by which the SCN exert control over circadian rhythms are not well understood. Most SCN efferents target the subparaventricular zone (SPZ), which resides just dorsal to the SCN [7, 8]. This output pathway has been proposed as a major component involved in the outflow for circadian regulation [9, 10]. We have examined the downstream pathway of the central clock by means of multiunit neural activity (MUA) [11] in freely moving mice. SCN neural activity is tightly coupled to environmental photic input and anticorrelated with MUA rhythm in the SPZ. In Clock mutant mice exhibiting attenuated circadian locomotor rhythmicity [12-14], MUA rhythmicity in the SCN and SPZ is similarly blunted. These results suggest that the SPZ plays a functional role in relaying circadian and photic signals to centers involved in generating behavioral activity.