PEROXISOMES CONTAIN DELTA(3,5),DELTA(2,4)-DIENOYL-COA ISOMERASE AND THUS POSSESS ALL ENZYMES REQUIRED FOR THE BETA-OXIDATION OF UNSATURATED FATTY-ACIDS BY A NOVEL REDUCTASE-DEPENDENT PATHWAY
PEROXISOMES CONTAIN DELTA(3,5),DELTA(2,4)-DIENOYL-COA ISOMERASE AND THUS POSSESS ALL ENZYMES REQUIRED FOR THE BETA-OXIDATION OF UNSATURATED FATTY-ACIDS BY A NOVEL REDUCTASE-DEPENDENT PATHWAY
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DOI：
10.1006/bbrc.1995.2428
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发表时间：
1995-10-04
影响因子：
3.1
通讯作者：
SCHULZ, H
中科院分区：
文献类型：
--
作者：
HE, XY;SHOUKRY, K;SCHULZ, H
通过测定大鼠肝脏中Delta（3，5），Delta（2，4）-二烯酰-CoA异构酶的亚细胞分布，证明过氧化物酶体中存在该酶。过氧化物酶体和线粒体形式的异构酶表现出类似的链长特异性，它们是同源的，如通过识别过氧化物酶体66-kDa酶的抗血清对线粒体32-kDa异构酶提出的。这份报告表明，过氧化物酶体含有所有的酶所需的β氧化的不饱和脂肪酸与奇数双键的一种新的途径，其中双键被还原性去除的NADPH依赖性的2，4-二烯酰辅酶A还原酶。(C)出版社：Academic Press
The presence of Delta(3,5),Delta(2,4)-dienoyl-CoA isomerase in peroxisomes was demonstrated by determining the subcellular distribution of this enzyme in rat liver. The peroxisomal and mitochondrial forms of the isomerase exhibit similar chain length specificities and they are homologous as indicated by the recognition of the peroxisomal 66-kDa enzyme by an antiserum raised against the mitochondrial 32-kDa isomerase. This report demonstrates that peroxisomes contain all enzymes required for the beta oxidation of unsaturated fatty acids with odd-numbered double bonds by a novel pathway in which double bonds are reductively removed by the NADPH-dependent 2,4-dienoyl-CoA reductase. (C) 1995 Academic Press,Inc.