A novel method for subarachnoid hemorrhage to induce vasospasm in mice
A novel method for subarachnoid hemorrhage to induce vasospasm in mice
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DOI：
10.1016/j.jneumeth.2009.06.027
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发表时间：
2009-10-15
影响因子：
3
通讯作者：
Provencio, J. Javier
中科院分区：
文献类型：
--
作者：
Altay, Tamer;Smithason, Saksith;Provencio, J. Javier
小鼠模型利用了可以在该物种中实现的遗传操作。目前有两种公认的蛛网膜下腔出血（SAH）和脑血管痉挛（CV）的小鼠模型。两者都是技术要求和劳动密集型的。在这项研究中，我们报告了一种可重复的和技术上可行的方法来诱导SAH，随后CV，在小鼠中。我们测试了这个模型在多个品系的小鼠，通常用于geneticmanipulation.Methods：SAH诱导C57 BL/6 NCr，FVB，129 S1，BalbC和SJL小鼠，体重28-32克，脑池内血管横断技术。在术后24小时用印度墨水灌注动物，并定量血管直径。脑切片进行苏木精-伊红染色（H&E），以寻找与CV一致的血管变化。结果：手术过程中或手术后无死亡。5种小鼠品系中有4种在术后24 h表现出显著CV，其特征在于靠近Willis环的大脑中动脉血管直径减小。组织学上，血管壁显示显着的肿胀和增厚，符合CVs.Conclusion：一种新的小鼠模型，诱导SAH的描述和测试中的几个小鼠品系。本研究中使用的五种菌株中有四种在诱导SAH后产生CV。该方法简单、直接、重复性好、死亡率低，适用于基因工程小鼠常用的背景品系。(C)2009年由Elsevier B. V.出版
Mouse models take advantage of genetic manipulations that can be achieved in this species. There are currently two accepted mouse models of subarachnoid hemorrhage (SAH) and cerebral vasospasm (CVs). Both are technically demanding and labor intensive. In this study,we report a reproducible and technically feasible method to induce SAH, and subsequently CVs, in mice. We tested this model in multiple strains of mice that are commonly used for genetic manipulation.Methods: SAH was induced in C57BL/6NCr, FVB, 129S1, BalbC and SJL mice, weighing 28-32 g, by an intracisternal vessel transection technique. Animals were perfused with India ink at 24 h postprocedure and vessel diameters were quantified. Brain slices were obtained for hematoxylin-eosin staining (H&E) to look for vascular changes consistent with CVs.Results: There was no mortality during or after the procedure. Four of the five mouse strains showed significant CVs at 24 h postprocedure characterized by decreased vessel diameter of the middle cerebral artery close to the Circle of Willis. Histologically, the vessel wall displayed significant corrugation and thickening, consistent with CVs.Conclusion: A novel mouse model to induce SAH is described and tested in several mouse strains. Four of the five strains used in this study developed CVs after the induction of SAH. The procedure is brief, straightforward, reproducible with low mortality, and applicable to commonly used background strains for genetically engineered mice. (C) 2009 Published by Elsevier B.V.