STUDIES ON REACTION OF FLUORESCAMINE WITH PRIMARY AMINES
STUDIES ON REACTION OF FLUORESCAMINE WITH PRIMARY AMINES
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DOI：
10.1016/0003-9861(74)90490-1
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发表时间：
1974-01-01
影响因子：
3.9
通讯作者：
UDENFRIEND, S
中科院分区：
文献类型：
--
作者：
DE BERNARDO, S;WEIGELE, M;UDENFRIEND, S
Fluorescamine 是一种用于伯胺荧光测定的有用试剂。荧光胺和伯胺之间的反应程度以及所得荧光团的荧光强度取决于 pH、溶剂组成和试剂浓度。这些变量的最佳值进一步取决于所研究的胺。已经研究了这些参数对代表性胺的荧光反应及其荧光衍生物的影响，并将结果报告于此。
Fluorescamine is a useful reagent for the fluorometric assay of primary amines. The extent of the reaction between fluorescamine and primary amines, as well as the fluorescence intensities of the resulting fluorophors depend on pH, solvent composition and reagent concentration. Optimum values for these variables further depend on the amine under study. The influence of these parameters on the fluorogenic reaction of representative amines, and on their fluorophoric derivatives has been investigated, and the results are reported here.