Complete mitochondrial genome of Nanorana pleskei (Amphibia: Anura: Dicroglossidae) and evolutionary characteristics
Complete mitochondrial genome of Nanorana pleskei (Amphibia: Anura: Dicroglossidae) and evolutionary characteristics
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Nanorana pleskei（两栖类：无尾目：Dicroglossidae）的完整线粒体基因组和进化特征
DOI：
10.1093/czoolo/57.6.785
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发表时间：
2011-01-01
期刊：
影响因子：
2.2
通讯作者：
Jiang, Jianping
中科院分区：
文献类型：
--
作者：
Chen, Guiying;Wang, Bin;Jiang, Jianping
它包括17,660个碱基对，包含13个蛋白质编码基因，2个rrna和23个trna。在该线粒体基因组中发现了tRNA（Met）基因的串联重复，两个tRNA（Met）基因的相似性为85.8%，是迄今为止测序的两栖动物线粒体基因组中最高的。根据基因组织，从145个两栖动物线粒体基因组中发现了24种类型。1型有108种，11型有11种，5型、16型、17型和20型各有2种，其余各有1种。在无尾动物中发现了15种类型，是Lissamphibia的三个目中最多样化的。我们对145个两栖动物线粒体基因组的11个蛋白编码基因序列进行系统发育分析，结果有力地支持了Lissamphibia及其三个目（Gymnophiona, Caudata, Anura）的单系性，其中亲缘关系为（(Gymnophiona (Caudata, Anura))）。基于系统发育树，1型被认为是两栖动物的祖先型，11型被认为是新蛙属的突触型。谱系间的基因重排提供了有意义的系统发育信息。LTPF tRNA基因簇的重排和ND5基因的易位仅在新巴塔achia中发现，支持该群体的单一性；同样的，tRNA（Met）基因的串联重复也只在Dicroglossidae中发现，这支持了该家族的单系性[Current Zoology 57(6): 785-805, 2011]。
The complete mitochondrial genome of Nanorana pleskei from the Qinghai-Tibet Plateau was sequenced. It includes 17,660 base pairs, containing 13 protein-coding genes, two rRNAs and 23 tRNAs. A tandem duplication of tRNA(Met) gene was found in this mitochondrial genome, and the similarity between the two tRNA(Met) genes is 85.8%, being the highest in amphibian mitochondrial genomes sequenced thus far. Based on gene organization, 24 types were found from 145 amphibian mitochondrial genomes. Type 1 was present in 108 species, type 11 in 11 species, types 5, 16, 17, and 20 each in two species, and the others each present in one species. Fifteen types were found in Anura, being the most diversity in three orders of the Lissamphibia. Our phylogenetic results using 11 protein-coding gene sequences of 145 amphibian mitochondrial genomes strongly support the monophyly of the Lissamphibia, as well as its three orders, the Gymnophiona, Caudata, and Anura, among which the relationships were ((Gymnophiona (Caudata, Anura)). Based on the phylogenetic trees, type 1 was recognized as the ancestral type for amphibians, and type 11 was the synapomorphic type for the Neobatrachia. Gene rearrangements among lineages provide meaningful phylogenetic information. The rearrangement of the LTPF tRNA gene cluster and the translocation of the ND5 gene only found in the Neobatrachia support the monophyly of this group; similarly, the tandem duplication of the tRNA(Met) genes only found in the Dicroglossidae support the monophyly of this family [Current Zoology 57 (6): 785-805, 2011].