EFFECTS OF FASTING AND HIBERNATION ON ION SECRETION IN GROUND-SQUIRREL INTESTINE
EFFECTS OF FASTING AND HIBERNATION ON ION SECRETION IN GROUND-SQUIRREL INTESTINE
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DOI：
10.1152/ajpregu.1992.263.6.r1203
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发表时间：
1992-12-01
影响因子：
--
通讯作者：
CAREY, HV
中科院分区：
文献类型：
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作者：
CAREY, HV
以黄鼠为研究对象，研究了禁食和冬眠对小肠分泌功能的影响。肌肉剥离片的空肠建立在通量室的挑战与各种分泌激动剂，短路电流（I（SC））的变化进行了记录。粘液湿重每厘米和隐窝尺寸没有受到影响，3天的快速活跃的松鼠，但绒毛的高度和面积显着减少，没有吃了6周以上的冬眠。组织电导率显着更大的禁食和冬眠的松鼠相比，喂养的动物。最大变化I（SC）诱发的粘膜下神经元的电刺激，标准化的serenum表面积，更大的空腹喂养或冬眠松鼠相比。当反应正常化的隐窝面积，神经诱发的变化，我（SC）是最大的冬眠。卡巴胆碱和5-羟色胺诱发的剂量依赖性的变化，在I（SC）是更大的在禁食相比，在所有浓度的喂养松鼠。组胺（100 μ M）和茶碱（1毫米）也产生了更大的增加，在I（SC）在禁食比在喂养松鼠。结果表明，为期3天的禁食，或延长冬眠的禁食，结果在地面松鼠空肠分泌能力增强。
Ground squirrels were used to study the effects of fasting and hibernation on small intestinal secretory function. Muscle-stripped sheets of jejunum set up in flux chambers were challenged with a variety of secretory agonists, and changes in short-circuit current (I(sc)) were recorded. Mucosal wet weights per centimeter and crypt dimensions were not affected by a 3-day fast in active squirrels, but villus height and area were significantly reduced in hibernators that had not eaten for over 6 wk. Tissue conductance was significantly greater in fasted and hibernating squirrels compared with fed animals. Maximal changes in I(sc) evoked by electrical stimulation of submucosal neurons, normalized to serosal surface area, were greater in fasted compared with fed or hibernating squirrels. When responses were normalized to crypt area, neurally evoked changes in I(sc) were greatest in the hibernators. Carbachol and serotonin evoked dose-dependent changes in I(sc) that were greater in fasted compared with fed squirrels at all concentrations. Histamine (100 muM) and theophylline (1 mM) also produced greater increases in I(sc) in fasted than in fed squirrels. The results suggest that a 3-day fast, or the extended fast of hibernation, results in enhanced secretory capacity in the ground squirrel jejunum.