The storage of globin mRNA during the inhibition of protein synthesis by heme deprivation.
The storage of globin mRNA during the inhibition of protein synthesis by heme deprivation.
复制标题
血红素剥夺抑制蛋白质合成期间珠蛋白 mRNA 的储存。
DOI：
10.1016/0006-291x(86)90357-8
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
London,IM
中科院分区：
文献类型：
--
作者：
Thomas,NS;Matts,RL;London,IM
血红素剥夺引起的网织红细胞及其裂解物中蛋白质合成的抑制在恢复最佳血红素浓度时是可逆的。抑制伴随着多聚核糖体的解聚和翻译机制组分的积累。通过测定加入未分级网织红细胞裂解物无细胞系统中的标记珠蛋白9S mRNA的命运，我们发现正常细胞mRNA在抑制过程中在20S和48S复合物中以及在80S核糖体二聚体OD 260峰之前沉淀的复合物中积累（称为≥80S复合物）1。20S和≥ 80S复合物是储存mRNA的主要库，如果恢复最佳血红素条件，则易于翻译。然而，在48S复合物中，mRNA保持非功能性，并且复合物是流产的，可能是由于Met的脱酰化。tRNAf.
The inhibition of protein synthesis in reticulocytes and their lysates caused by heme-deprivation is reversible on restoration of an optimal heme concentration. Inhibition is accompanied by the disaggregation of polyribosomes and the accumulation of components of the translational mechanism. By determining the fate of labeled globin 9S mRNA added to an unfractionated reticulocyte lysate cell-free system, we find that normal cellular mRNA accumulates during inhibition in 20S and 48S complexes and in a complex which sediments just ahead of the 80S ribosome dimer OD260peak (designated as ≥80S complex)1. The 20S and ≥ 80S complexes are the major pools of stored mRNA which is readily translated if optimal heme conditions are restored. In the 48S complex, however, the mRNA remains non-functional, and the complex is abortive, probably as a result of deacylation of the Met. tRNAf.