Associations Between Infiltrating Lymphocyte Subsets and Hepatocellular Carcinoma
Associations Between Infiltrating Lymphocyte Subsets and Hepatocellular Carcinoma
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浸润淋巴细胞亚群与肝细胞癌之间的关联
DOI：
10.7314/apjcp.2012.13.11.5909
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Zhao, Liang
中科院分区：
文献类型：
--
作者：
Guo, Cun-Li;Yang, Hai-Chao;Zhao, Liang
目的：我们旨在分析HCC和非肿瘤组织中肿瘤浸润淋巴细胞（til）和非肿瘤浸润淋巴细胞（NILs）的表型，并评估这些细胞的变化与HCC预后的关系。方法：从HCC及相应的非肿瘤组织中分离淋巴细胞，采用流式细胞术检测。为了比较，分析临床参数。结果：与非肿瘤组织相比，肿瘤组织中NK、NKT和CD8+T细胞浸润强度较低。TILs的CD4+CD25+Foxp3+调节性T细胞（Treg cells）浸润强度高于NILs。在HCC免疫微环境中，Treg细胞的流行与CD8+T淋巴细胞的减少有关。NK细胞和CD8+T细胞在转移期小于12个月的HCC患者的TILs中出现的频率低于未发生转移的患者。然而，Treg细胞的频率高于无转移的细胞。结论：HCC肿瘤组织中CD8+T、NK、NKT细胞及Treg细胞的频率与患者生存率有显著相关性，可作为HCC预后的预测指标。
Aims: We aimed to analyze the phenotype of tumor-infiltrating lymphocytes (TILs) and non-tumor infiltrating lymphocytes (NILs) in HCC and non-tumor tissues, and evaluate relationships between changes in these cells and the prognosis of HCC. Methods: Lymphocytes were isolated from HCC and corresponding non-tumor tissues and tested by flow cytometry. For comparison, clinical parameters were analyzed. Results: Compared with the non-tumor tissue, tumor tissue had a lower intensity of NK, NKT and CD8+T cell infiltration. TILs had higher intensity of CD4+CD25+Foxp3+regulatory T cell (Treg cells) infiltration compared with that in NILs. The prevalence of Treg cells was associated with fewer CD8+T lymphocytes in the HCC immune microenvironment. The frequencies of NK cells and CD8+T cells in TILs of HCC patients with metastasis less than 12 months were lower than those without metastasis. However, the frequency of Treg cells was higher than those without metastasis. Conclusion: These results suggest that the frequencies of CD8+T, NK and NKT cells as well as Treg cells in the tumor tissue of HCC are significantly associated with patient survival, and could be applied as predictive indicators for HCC prognosis.