An enhanced linkage map of the sheep genome comprising more than 1000 loci
An enhanced linkage map of the sheep genome comprising more than 1000 loci
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DOI：
10.1101/gr.gr-1350r
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发表时间：
2001-07-01
期刊：
影响因子：
7
通讯作者：
van Hest, B
中科院分区：
文献类型：
--
作者：
Maddox, JF;Davies, KP;van Hest, B
绵羊基因组的中密度连锁图已经开发出来。产生了550个新位点的标记数据，并与以前的绵羊连锁图合并。新图谱包括1093个标记，代表1062个独特的基因座（941个匿名基因座，121个基因）和跨度3500 cM（性别平均）的常染色体和132 cM（女性）的X染色体。常染色体基因座之间的平均间距为3.4 cM，高度多态性（多态信息含量（PIC）大于或等于0.7）常染色体基因座之间的平均间距为8.3 cM。标记之间的最大间隙为32.5cM，并且基因座之间或基因座从染色体末端缺失的区域之间> 20cM的间隙的数目已从先前图谱中的40个减少到6个。573个基因座可以在框架图上排序，几率>1000：1。绵羊连锁图与牛和山羊连锁图都有很强的联系。定位在绵羊连锁图上的572个位点也通过连锁分析在牛中定位，定位在绵羊连锁图上的209个位点也被定位在山羊连锁图上。反刍动物的连锁图谱的检查表明，目前的绵羊连锁图谱的基因组覆盖率是可比的牛的地图。绵羊基因图谱为国际绵羊、牛和山羊基因图谱研究提供了宝贵的资源。
A medium-density linkage map of the ovine genome has been developed. Marker data for 550 new loci were generated and merged with the previous sheep linkage map. The new map comprises 1093 markers representing 1062 unique loci (941 anonymous loci, 121 genes) and spans 3500 cM (sex-averaged) For the autosomes and 132 cM (female) on the X chromosome. There is an average spacing of 3.4 cM between autosomal loci and 8.3 cM between highly polymorphic [polymorphic information content (PIC) greater than or equal to 0.7] autosomal loci. The largest gap between markers is 32.5 cM, and the number of gaps of >20 cM between loci, or regions where loci are missing From chromosome ends, has been reduced from 40 in the previous map to 6. Five hundred and seventy-three of the loci can be ordered on a framework map with odds of >1000:1. The sheep linkage map contains strong links to both the cattle and goat maps. Five hundred and seventy-two of the loci positioned on the sheep linkage map have also been mapped by linkage analysis in cattle, and 209 of the loci mapped on the sheep linkage map have also been placed on the goat linkage map. Inspection of ruminant linkage maps indicates that the genomic coverage by the current sheep linkage map is comparable to that of the available cattle maps. The sheep map provides a valuable resource to the international sheep, cattle, and goat gene mapping community.