Gentamicin- and neomycin-resistant mutants of Escherichia coli K-12 cross-resistant to nitrofurans.
Gentamicin- and neomycin-resistant mutants of Escherichia coli K-12 cross-resistant to nitrofurans.
复制标题
大肠杆菌 K-12 的庆大霉素和新霉素耐药突变体对硝基呋喃具有交叉耐药性。
DOI：
10.1093/jac/13.6.567
复制
发表时间：
1984
影响因子：
5.2
通讯作者：
A. Breeze
中科院分区：
文献类型：
--
作者：
E. E. Obaseiki;A. Breeze
分离出大肠杆菌K-12的庆大霉素和新霉素抗性突变体，其中一些对硝基呋喃、氯酸盐、P1和λ噬菌体以及所有测试的氨基糖苷类抗生素具有抗性。以前分离的硝基呋喃耐药突变体没有交叉耐药的测试氨基糖苷类，氯酸盐和噬菌体。氨基糖苷类抗生素耐药突变株表现出交叉耐药，增强膜Mg 2 +-ATP酶和降低周质碱性磷酸酶活性相比，他们的父母敏感菌株。突变体也有缺陷的糖发酵，硝酸盐和亚硝酸盐的还原，并在甲酸脱氢酶活性。初步遗传学研究表明，该多效性突变可能位于E. coliK-12染色体连锁图谱。据推测，这种突变体与总膜的变化有受损的积累氨基糖苷类和硝基呋喃类抗生素。
Gentamicin and neomycin-resistant mutants of Escherichia coli K-12 were isolated some of which were resistant to nitrofurans, chlorate, P1 and lambda bacteriophages as well as to all aminoglycoside antibiotics tested. Previously isolated nitrofuran-resistant mutants were not cross-resistant to the tested aminoglycosides, chlorate and bacteriophages. The aminoglycoside-resistant mutants exhibiting cross-resistance had enhanced membrane Mg2+-ATPase and decreased periplasmic alkaline phosphatase activity compared to their parent sensitive strains. The mutants were also defective in the fermentation of sugars, reduction of nitrate and nitrite, and in formate dehydrogenase activity. Preliminary genetic studies indicated that the pleiotropic mutation might be located in the upper left quadrant of the E. coli K-12 chromosome linkage map. It is postulated that such mutants with gross membrane alterations have impaired accumulation of aminoglycoside and nitrofuran antibiotics.