The RegB/RegA two-component regulatory system controls synthesis of photosynthesis and respiratory electron transfer components in Rhodobacter capsulatus
The RegB/RegA two-component regulatory system controls synthesis of photosynthesis and respiratory electron transfer components in Rhodobacter capsulatus
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DOI：
10.1006/jmbi.2001.4652
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发表时间：
2001-05-25
影响因子：
5.6
通讯作者：
Bauer, CE
中科院分区：
文献类型：
--
作者：
Swem, LR;Elsen, S;Bauer, CE
最近，我们证明了RegB/RegA双组分调节系统从Rhodobacter capsulatus的功能作为一个全球性的调节代谢过程，无论是产生或消耗还原当量。例如，RegB/RegA系统控制光合作用和氢利用等能量产生过程的表达。此外，RegB/RegA还控制利用还原当量的氮和碳固定途径。在这里，我们结合使用DNA酶I保护和基于质粒的报告基因表达研究来证明RegA直接控制细胞色素cbb（3）和泛醇氧化酶的合成，这些酶在分支呼吸链中充当末端电子受体。我们还表明，RegA控制细胞色素c（2），c（y），和细胞色素be，复杂的参与光合和呼吸电子传递事件的表达。这些数据提供的证据表明，RegB/RegA双组分系统在控制影响荚膜红细菌中还原当量的许多过程的合成中具有重要作用。(C)北京：科学出版社.
Recently, we demonstrated that the RegB/RegA two-component regulatory system from Rhodobacter capsulatus functions as a global regulator of metabolic processes that either generate or consume reducing equivalents. For example, the RegB/RegA system controls expression of such energy generating processes as photosynthesis and hydrogen utilization. In addition, RegB/RegA also control nitrogen and carbon fixation pathways that utilize reducing equivalents. Here, we use a combination of DNase I protection and plasmid-based reporter expression studies to demonstrate that RegA directly controls synthesis of cytochrome cbb(3) and ubiquinol oxidases that function as terminal electron accepters in a branched respiratory chain. We also demonstrate that RegA controls expression of cytochromes c(2), c(y), and the cytochrome be, complex that are involved in both photosynthetic and respiratory electron transfer events. These data provide evidence that the RegB/RegA two-component system has a major role in controlling the synthesis of numerous processes that affect reducing equivalents in Rhodobacter capsulatus. (C) 2001 Academic Press.