UNDERMODIFICATION IN THE 1ST POSITION OF THE ANTICODON OF SUPG-TRANSFER RNA REDUCES TRANSLATIONAL EFFICIENCY
UNDERMODIFICATION IN THE 1ST POSITION OF THE ANTICODON OF SUPG-TRANSFER RNA REDUCES TRANSLATIONAL EFFICIENCY
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DOI：
10.1007/bf00328050
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
BJORK, GR
中科院分区：
文献类型：
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作者：
HAGERVALL, TG;BJORK, GR
利用大肠杆菌突变株trmC 1和trmC 2研究了5-甲氨甲基-2-硫代尿苷（mnm 5s 2U）的功能。对谷氨酰胺、谷氨酸和赖氨酸具有特异性的转移RNA以及来自赖氨酸tRNA的特异性赭石抑制因子。**图形 **。由supG等位基因编码）在位置34（摆动位置）含有该修饰的核苷。在2个trmC突变体中存在两种不同的未修饰的mnm 5s 2U衍生物，这表明2个突变影响2种不同的酶活性。使用lacI-Z融合系统，发现在trmC突变体中supG介导的抑制效率降低至野生型值的30-90%。突变体中修饰缺陷的supG-tRNA对密码子上下文的敏感性高于正常情况。**图形 **。
Two mutants of Escherichia coli, trmC1 and trmC2, which are defective in the synthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) were utilized to study the function of this complex modified nucleoside. Transfer RNA specific for glutamine, glutamic acid and lysine and a specific ochre suppressor derived from lysine tRNA .**GRAPHIC**. encoded by the supG allele), contain this modified nucleoside at position 34 (the wobble position). Two different undermodified derivatives of mnm5s2U were present in the 2 trmC mutants, which suggests that the 2 mutations affect 2 different enzymatic activities. Using the lacI-Z fusion system it was found that the efficiency of supG-mediated suppression was reduced to 30-90% of the wild-type value in the trmC mutants. The modification-deficient supG-tRNA in the mutants showed a higher sensitivity to codon context than the normal .**GRAPHIC**.