The phage lambda gene Q transcription antiterminator binds DNA in the late gene promoter as it modifies RNA polymerase.
The phage lambda gene Q transcription antiterminator binds DNA in the late gene promoter as it modifies RNA polymerase.
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噬菌体 lambda 基因 Q 转录抗终止子在修饰 RNA 聚合酶时与晚期基因启动子中的 DNA 结合。
DOI：
10.1016/0092-8674(92)90639-t
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发表时间：
1992
期刊：
影响因子：
64.5
通讯作者：
Roberts,JW
中科院分区：
文献类型：
--
作者：
Yarnell,WS;Roberts,JW
噬菌体I.基因Q转录抗终止子在噬菌体晚期基因启动子转录物的nt+ 16和nt + 17处延伸的延长暂停期间修饰RNA聚合酶。我们在这里表明，Q结合一个特定的DNA位点之间的-10和-36元件的启动子，因为它与酶的相互作用。我们表明，暂停必须反映一个专门的延伸结构，是接受修改0，因为Q不结合RNA聚合酶停止后，转录16个核苷酸的突变体DNA，不编码的自然暂停人工。足迹分析表明，RNA聚合酶在暂停复合物中与Q结合的区域中的DNA进行独特的相互作用; Q的结合反过来改变了Q结合位点和转录泡中的足迹。Q与暂停的转录复合物的结合由转录因子NusA稳定，如从H Q介导的抗终止对NusA的依赖所预期的。
The bacteriophage I. gene Q transcription antiterminator modifies RNA polymerase during an extended pause in elongation at nt+ 16 and+ 17 of the phage late gene promoter transcript. We show here that Q binds a specific DNA site between the-10 and-36 elements of the promoter as it Interacts with the enzyme. We show that the pause must reflect a specialized elongation structure that is receptive to modification by 0, because Q does not bind to RNA polymerase stopped artificially after transcribing 16 nt of mutant DNA that does not encode the natural pause. Footprlntlng shows that RNA polymerase in the paused complex makes distinctive interactions with DNA in the region where Q binds; binding of Q, in turn, changes the footprint both at the Q-binding site and in the transcription bubble. Binding of Q to the paused transcription complex is stabilized by the transcription factor NusA, as expected from the dependence of h Q-mediated antitermination on NusA.