Early oligodendrocyte progenitor cells in the human fetal telencephalon
Early oligodendrocyte progenitor cells in the human fetal telencephalon
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DOI：
10.1002/glia.10140
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发表时间：
2003-01-15
期刊：
影响因子：
6.2
通讯作者：
Zecevic, N
中科院分区：
文献类型：
--
作者：
Rakic, S;Zecevic, N
少突胶质细胞是中枢神经系统中产生髓鞘的细胞，占人脑细胞总数的很大一部分。使用细胞特异性标记和抗体的腹侧同源结构域转录因子，NKX2.1和DLX 2，我们在这里显示，在人类端脑的早期少突胶质细胞祖细胞（OPC）的亚群可能起源于神经节隆起（GE）。DLX 2标记的OPC形成了一个轮廓清晰的细胞流，通过前皮质SVZ将GE脑室下区（SVZ）连接到皮质中间区。该细胞群被早期OPCs标记物PDGFR α、Olig 1和NG 2标记，而不是被神经元、星形胶质细胞或晚期OPCs标记物标记。有趣的是，大量的CD 68（+）小胶质细胞/巨噬细胞、巢蛋白（+）神经干细胞和CD 34（+）造血干细胞（HSC）也存在于GE流和皮质SVZ中。这些细胞可以用DLX 2以及早期OPCs标记物共标记。早期OPC的一个单独的亚群，存在于GE和皮质SVZ，不表达DLX 2或CD 68。这些发现表明，不同的早期OPCs亚群，具有不同的转录因子和细胞特异性标记，存在于人类前脑。这些亚群可能有不同的起源;一个可能起源于皮质SVZ，而其他可能来自GE和/或CNS外的造血干细胞。
Oligodendrocytes, the myelin-producing cells in the central nervous system, represent a large portion of the total number of cells in the human brain. Using cell-specific markers and antibodies to ventral homeodomain transcription factors, NKX2.1 and DLX2, we show here that a subpopulation of early oligodendrocyte progenitor cells (OPCs) in the human telencephalon may originate in the ganglionic eminence (GE). DLX2-labeled OPCs form a well-delineated stream of cells connecting the GE subventricular zone (SVZ) to the cortical intermediate zone through the anterior cortical SVZ. This population of cells is labeled by early OPCs markers, PDGFRalpha, Olig1, and NG2, and not with either neuronal, astrocyte, or late OPCs markers. Intriguingly, numerous CD68(+) microglia/macrophages, nestin(+) neural stem cells, and CD34(+) hematopoietic stem cells (HSCs) are also present in both the GE stream and the cortical SVZ. These cells could be colabeled with DLX2 as well as early OPCs markers. A separate subpopulation of early OPCs, present in the GE and cortical SVZ, did not express either DLX2 or CD68. These findings suggest that different subpopulations of early OPCs, characterized with different sets of transcription factors and cell-specific markers, are present in human forebrain. These subpopulations may have different origins; one may originate in the cortical SVZ, while others may come from the GE and/or outside the CNS as hematopoietic stem cells.