Avidity of immunoglobulin G directed against human cytomegalovirus during primary and secondary infections in immunocompetent and immunocompromised subjects
Avidity of immunoglobulin G directed against human cytomegalovirus during primary and secondary infections in immunocompetent and immunocompromised subjects
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DOI：
10.1128/cdli.4.4.469-473.1997
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发表时间：
1997-07-01
期刊：
影响因子：
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通讯作者：
Landini, MP
中科院分区：
文献类型：
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作者：
Lazzarotto, T;Spezzacatena, P;Landini, MP
原发性人巨细胞病毒（HCMV）感染的诊断完全通过血清学检测完成。在所有可能的检测方法中，免疫球蛋白G （IgG）贪婪度的测定是研究最少的方法之一。在这项工作中，我们使用市售试剂盒检测了336份血清样本中抗HCMV IgG的亲和力，这些血清样本来自病毒学证实的HCMV原发性或非原发性感染的孕妇和移植受体，以及潜在感染的献血者。我们的研究结果表明，抗HCMV IgG抗体检测可区分孕妇和实体器官移植受者的原发性和非原发性HCMV感染。事实上，88.6%的原发性感染和无继发性感染显示对HCMV的低亲和力IgG。特别是，在免疫功能低下和免疫功能正常的受试者出现症状后18至20周内，低IgG贪婪度是原发性感染的标志。
Diagnosis of primary human cytomegalovirus (HCMV) infection is accomplished exclusively by serologic testing. Among the possible methods, the determination of immunoglobulin G (IgG) avidity is one of the least explored. In this work, we used a commercially available kit to test anti-HCMV IgG avidity in 336 serum samples from pregnant women and transplant recipients undergoing virologically proven HCMV primary or nonprimary infections and from latently infected blood donors. Our results demonstrate that the anti-HCMV IgG avidity test differentiates primary from nonprimary HCMV infections in both pregnant women and solid organ transplant recipients. In fact, 88.6% of primary infections and no secondary infections showed low-avidity IgG to HCMV. In particular, low IgG avidity is a marker of primary infection for 18 to 20 weeks after onset of symptoms in both immunocompromised and immunocompetent subjects.