Morphological effect of oscillating magnetic nanoparticles in killing tumor cells.
Morphological effect of oscillating magnetic nanoparticles in killing tumor cells.
复制标题
振荡磁性纳米颗粒在杀死肿瘤细胞中的形态学作用。
DOI：
10.1186/1556-276x-9-195
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发表时间：
2014
影响因子：
--
通讯作者：
Shi H
中科院分区：
文献类型：
--
作者：
Cheng D;Li X;Zhang G;Shi H
首次利用低功率低频交变磁场（AMF）对球形和棒状氧化铁磁性纳米粒子（MNP）进行强迫振荡，并在体外对癌细胞进行杀伤。在负载人宫颈癌细胞系（HeLa）后，然后暴露于35-kHz AMF，MNP机械地破坏细胞膜和细胞质，降低细胞活力。结果发现，MNP的浓度和形态显着影响振荡MNP的细胞杀伤效率。在这项初步研究中，当HeLa细胞与100 μg/mL棒状MNP预孵育时，MTT法显示AMF作用2 h后细胞活力下降30.9%，而球形MNP作用2 h后细胞活力下降11.7（sMNP，直径200 ± 50 nm）用于研究。台盼蓝染色结果显示，AMF作用2 h后，39.5%的rMNP负载细胞和15.1%的sMNP负载细胞染色，细胞存活率明显下降。还有趣的是，发现在较高（500 μg/mL）或较低（20 μg/mL）浓度的MNP下杀死肿瘤细胞的效率低于在100 μg/mL浓度下实现的效率。总之，当暴露于AMF时，相对不对称形态的杆状MNP可以比球形MNP更有效地杀死癌细胞，这是由于它们的机械振荡。
Forced oscillation of spherical and rod-shaped iron oxide magnetic nanoparticles (MNPs) via low-power and low-frequency alternating magnetic field (AMF) was firstly used to kill cancer cells in vitro. After being loaded by human cervical cancer cells line (HeLa) and then exposed to a 35-kHz AMF, MNPs mechanically damaged cell membranes and cytoplasm, decreasing the cell viability. It was found that the concentration and morphology of the MNPs significantly influenced the cell-killing efficiency of oscillating MNPs. In this preliminary study, when HeLa cells were pre-incubated with 100 μg/mL rod-shaped MNPs (rMNP, length of 200 ± 50 nm and diameter of 50 to 120 nm) for 20 h, MTT assay proved that the cell viability decreased by 30.9% after being exposed to AMF for 2 h, while the cell viability decreased by 11.7% if spherical MNPs (sMNP, diameter of 200 ± 50 nm) were used for investigation. Furthermore, the morphological effect of MNPs on cell viability was confirmed by trypan blue assay: 39.5% rMNP-loaded cells and 15.1% sMNP-loaded cells were stained after being exposed to AMF for 2 h. It was also interesting to find that killing tumor cells at either higher (500 μg/mL) or lower (20 μg/mL) concentration of MNPs was less efficient than that achieved at 100 μg/mL concentration. In conclusion, the relatively asymmetric morphological rod-shaped MNPs can kill cancer cells more effectively than spherical MNPs when being exposed to AMF by virtue of their mechanical oscillations.