Morphological and molecular variation in Mitchella undulata, with special reference to the systematic treatment of the dwarf form from Yakushima
Morphological and molecular variation in Mitchella undulata, with special reference to the systematic treatment of the dwarf form from Yakushima
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DOI：
10.1007/s10265-003-0105-7
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发表时间：
2003-06
影响因子：
2.8
通讯作者：
J. Yokoyama;T. Fukuda;H. Tsukaya
中科院分区：
文献类型：
--
作者：
J. Yokoyama;T. Fukuda;H. Tsukaya
波纹米切尔藻的形态和分子变异。的遗传基础，以评价识别矮秆品种，M。undulatavar.minorMasamune. M.叶片大小变异较大。在正常和矮秆品种之间，没有发现明显的形态不连续。相反，从太平洋到日本海的一个弱的渐变群被发现。叶片的解剖学观察表明，叶片大小的巨大变化可以归因于叶细胞数量的变化，而不是细胞大小的差异。基于rDNA内转录间隔区序列的分子生物学分析表明，M有两种主要的基因型。波状的，由于额外的替换或假定的重组而导致的单倍型的微小变化。屋久岛的侏儒型既没有遗传上的统一，也没有明显地与其他种群分化。从这些结果中，我们得出结论：M.波浪形应按形式级处理。
Morphological and molecular variation inMitchella undulataSiebold et Zucc. was examined to evaluate the genetic basis for recognizing the dwarf variety,M. undulatavar.minorMasamune. Considerable variation in leaf size inM. undulata, but no obvious morphological discontinuities, were found between the normal and dwarf varieties. Instead, a weak cline running from the Pacific Ocean to the Sea of Japan was found. Anatomical observations of leaf blades revealed that the large variation in leaf size can be attributed to variation in the number of leaf cells and not to differences in cell size. A molecular analysis based on sequences of rDNA internal transcribed spacer regions indicated that there were two major genotypes inM. undulatawith minor variation in haplotypes resulting from additional substitutions or putative recombination. The dwarf form from Yakushima was neither genetically uniform nor apparently differentiated from other populations. From these results, we conclude that the dwarf form ofM. undulatashould be treated at the rank of forma.