Construction of a BAC library and its application to the identification of simple sequence repeats in peach [Prunus persica (L.) Batsch]
Construction of a BAC library and its application to the identification of simple sequence repeats in peach [Prunus persica (L.) Batsch]
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DOI：
10.1007/s00122-002-0967-4
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发表时间：
2002-12-01
影响因子：
5.4
通讯作者：
Abbott, AG
中科院分区：
文献类型：
--
作者：
Georgi, LL;Wang, Y;Abbott, AG
蔷薇科包含许多经济上重要的作物物种，但它们的基因组没有得到很好的表征，比较遗传图谱远远落后于其他科。为了便于基因组比较和基因发现在蔷薇科，我们已经开始了桃的基因组资源的开发作为模式基因组为这个家庭。首先，我们开发了一种简化的，具有成本效益的方法来构建BAC库，特别适合于相对较小的经济重要性的植物物种。其次，我们利用该文库研究了桃中SSR的丰度和局部分布。我们的研究结果表明，微卫星位点在当地比以前估计的更丰富，BAC测序结果表明，微卫星重复序列不是随机分布在桃基因组的基因区域内。这使得通过与BAC克隆杂交，甚至通过BAC克隆的随机测序来鉴定桃中的SSR相对容易，这些BAC克隆事先不知道含有SSR。
The Rosaceae contains many economically important crop species, but their genomes are not well characterized, and comparative genetic mapping lags well behind that of other families. To facilitate genome comparisons and gene discovery in the Rosaceae, we have begun the development of genomic resources for peach as the model genome for this family. First, we developed a simplified, cost-effective method for constructing BAC libraries, particularly appropriate for plant species of relatively minor economic importance. Second, we used the library to investigate the abundance and local distribution of simple sequence repeats (SSRs) in peach. Our results indicate that microsatellite loci are locally much more highly abundant than previously estimated, and BAC sequencing results suggest that microsatellite repeats are not randomly distributed within gene-containing regions of the peach genome. This makes it relatively easy to identify SSRs in peach by hybridization to BAC clones, and even by random sequencing of BAC clones, not known a priori to contain SSRs.