Advances in farm animal transgenesis
Advances in farm animal transgenesis
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DOI：
10.1016/j.prevetmed.2011.04.009
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发表时间：
2011-11-01
影响因子：
2.6
通讯作者：
Niemann, Heiner
中科院分区：
文献类型：
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作者：
Kues, Wilfried A.;Niemann, Heiner
1985年，将外源DNA微量注射到受精卵原核中，培育出了第一批转基因家畜。这是20多年来的首选方法，但现在有更有效的方案，包括体细胞核移植和慢病毒转基因。典型的应用包括胴体成分、泌乳性能和羊毛生产，以及增强抗病能力和减少环境影响。用于生物医学应用的转基因农场动物生产已被广泛接受。2006年，欧洲药品管理局（EMA）批准了首个在转基因山羊乳腺中生产的重组药物蛋白——抗凝血酶的商业化。随着各种农场动物基因组测序项目的完成，利用慢病毒载体、小干扰核糖核酸、巨核酸酶、锌指核酸酶和转座子等新兴工具进行精确的遗传修饰已经成为可能。我们预计，农场动物的基因改造将有助于应对农业生产中的全球挑战，并将开辟生物医学的新视野。(C) 2011 Elsevier B.V.版权所有
The first transgenic livestock were produced in 1985 by microinjection of foreign DNA into zygotic pronuclei. This was the method of choice for more than 20 years, but more efficient protocols are now available, including somatic cell nuclear transfer and lentiviral transgenesis. Typical applications include carcass composition, lactational performance and wool production, as well as enhanced disease resistance and reduced environmental impact. Transgenic farm animal production for biomedical applications has found broad acceptance. In 2006 the European Medicines Agency (EMA) approved commercialization of the first recombinant pharmaceutical protein, antithrombin, produced in the mammary gland of transgenic goats. As the genome sequencing projects for various farm animal species are completed, it has become feasible to perform precise genetic modifications employing the emerging tools of lentiviral vectors, small interfering ribonucleic acids, meganucleases, zinc finger nucleases and transposons. We anticipate that genetic modification of farm animals will be instrumental in meeting global challenges in agricultural production and will open new horizons in biomedicine. (C) 2011 Elsevier B.V. All rights reserved.