STIMULATION OF BONE-FORMATION INVIVO BY TRANSFORMING GROWTH-FACTOR-BETA - REMODELING OF WOVEN BONE AND LACK OF INHIBITION BY INDOMETHACIN
STIMULATION OF BONE-FORMATION INVIVO BY TRANSFORMING GROWTH-FACTOR-BETA - REMODELING OF WOVEN BONE AND LACK OF INHIBITION BY INDOMETHACIN
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DOI：
10.1016/8756-3282(90)90083-b
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发表时间：
1990-01-01
期刊：
影响因子：
4.1
通讯作者：
TRECHSEL, U
中科院分区：
文献类型：
--
作者：
MACKIE, EJ;TRECHSEL, U
转化生长因子的局部效应。（tgf . β）对生长小鼠骨的影响。吲哚美辛对tgf . β的影响。还检查了效果。每天注射五次tgf。-1或-2在7周龄小鼠的额骨和顶骨上皮下注射。在用tgf治疗的动物中。单独或tgf。第19天处死（第1天=第1天tgf . β）。注射)，额骨和顶骨联合骨较对照组重，额骨较对照组厚。第4天，tgf - β在颅骨骨膜可见多层分化成骨细胞。对待动物。第8天，tgf . β颅骨内可见高度血管化的骨膜编织骨。对待动物。第19天（末次注射后第14天）编织骨已部分重建为含骨髓的板层骨，且仍在积极重建中。与tgf . β同时处理的动物骨骼的组织学外观。和吲哚美辛与用tgf - β治疗的动物相同。而tgf . β和-1和tgf - β -2。因此,TGF.beta。刺激骨形成在体内是一个长期的影响，似乎是独立于前列腺素。tgf . β切片破骨细胞计数。尽管在这个阶段对成骨细胞的影响在形态学上已经很明显，但在第4天，处理过的动物没有升高。tgf . β中破骨细胞计数。在第8天和第19天，处理后的骨量显著高于对照组。这些结果表明tgf . β。不直接影响体内骨吸收，但破骨细胞被募集以重塑tgf . β。-诱导编织骨。
Local effects of transforming growth factor-.beta. (TGF.beta.) on bone have been investigated in growing mice. The influence of indomethacin on TGF.beta. effects was also examined. Five daily injections of TGF.beta.-1 or -2 were administered subcutaneously over the frontal and parietal bones of seven-week-old mice. In animals treated with TGF.beta. alone or TGF.beta. and indomethacin, then killed on day 19 (day 1 = day of first TGF.beta. injection), the combined frontal and parietal bones were heavier and the frontal bones were thicker than in controls. On day 4, multiple layers of differentiating osteoblasts were visible in the periosteum of calvariae from TGF.beta.-treated animals. On day 8, highly vascularised periosteal woven bone was seen in the calvariae of TGF.beta.-treated animals. On day 19 (14 days after the last injection) the woven bone had been partially remodeled into lamellar bone containing bone marrow, and was still actively being remodeled. The histological appearance of bones from animals treated concurrently with TGF.beta. and indomethacin was identical to that of animals treated with TGF.beta. alone, and there appeared to be no difference between TGF.beta.-1 and TGF.beta.-2. Thus, TGF.beta.-stimulated bone formation in vivo is a long term effect that appears to be independent of prostaglandins. Osteoclast counts in sections of TGF.beta.-treated animals were not elevated on day 4, although at this stage effects on osteoblastic cells were already morphologically apparent. Osteoclast counts in TGF.beta.-treated bones were significantly increased over control values on days 8 and 19. These results suggest that TGF.beta. does not directly influence bone resorption in vivo, but that osteoclasts are recruited in order to remodel TGF.beta.-induced woven bone.