The association of vesicular contents and its effects on release.
The association of vesicular contents and its effects on release.
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囊泡内容物的关联及其对释放的影响。
DOI：
10.1111/j.1749-6632.2002.tb04540.x
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发表时间：
2002
影响因子：
5.2
通讯作者：
Catahan,Rebecca
中科院分区：
文献类型：
--
作者：
Wightman,RMark;Troyer,KevinP;Mundorf,MichelleL;Catahan,Rebecca
翻译后摘要：与碳纤维电极的儿茶酚胺的氧化可用于监测胞吐在单细胞水平在各种不同类型的细胞。这些测量允许跟踪单个囊泡的释放，并揭示了有关释放和储存之间耦合的几个独特方面。嗜铬细胞中的囊泡成分的强关联决定了囊泡内容物挤出的时间过程。此外，释放正常储存在囊泡内的Ca2+可以促进胞吐作用，而无需外部Ca2+源。
Abstract:The oxidation of catecholamines with a carbon‐fiber electrode can be used to monitor exocytosis at the single cell level at a variety of different types of cells. These measurements allow release to be followed from individual vesicles and have revealed several unique aspects concerning the coupling between release and storage. The strong association of the vesicular components in chromaffin cells dictates the time course of extrusion of the vesicle contents. Furthermore, liberation of the Ca2+normally stored within the vesicles can promote exocytosis without an external Ca2+source.