MRC2014: Extensions to the MRC format header for electron cryo-microscopy and tomography.
MRC2014: Extensions to the MRC format header for electron cryo-microscopy and tomography.
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DOI：
10.1016/j.jsb.2015.04.002
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发表时间：
2015-11
影响因子：
3
通讯作者：
Winn M
中科院分区：
文献类型：
--
作者：
Cheng A;Henderson R;Mastronarde D;Ludtke SJ;Schoenmakers RH;Short J;Marabini R;Dallakyan S;Agard D;Winn M
MRC二进制文件格式广泛应用于三维电子显微镜领域，用于存储图像和体数据。文件包含一个描述所保存数据类型的头，以及其他重要的元数据。为了响应电子显微镜技术的进步，出现了许多文件格式的变体，其中包含有用的附加数据，但限制了不同软件包之间的互操作性。经过广泛的讨论，代表该领域领先软件包的作者提出了一组MRC格式标准的扩展，旨在适应这些变体，同时恢复互操作性。MRC格式相当于用于大分子晶体学的CCP4套件中使用的映射格式，并且该提案还保持了与晶体学软件的互操作性。本技术说明描述了建议的扩展，并作为标准的参考。
The MRC binary file format is widely used in the three-dimensional electron microscopy field for storing image and volume data. Files contain a header which describes the kind of data held, together with other important metadata. In response to advances in electron microscopy techniques, a number of variants to the file format have emerged which contain useful additional data, but which limit interoperability between different software packages. Following extensive discussions, the authors, who represent leading software packages in the field, propose a set of extensions to the MRC format standard designed to accommodate these variants, while restoring interoperability. The MRC format is equivalent to the map format used in the CCP4 suite for macromolecular crystallography, and the proposal also maintains interoperability with crystallography software. This Technical Note describes the proposed extensions, and serves as a reference for the standard.