Drosophila syntaxin is required for cell viability and may function in membrane formation and stabilization.
Drosophila syntaxin is required for cell viability and may function in membrane formation and stabilization.
复制标题
果蝇突触蛋白是细胞活力所必需的，并且可能在膜形成和稳定中发挥作用。
作者：
Karen L. Schulze;Hugo J. Bellen
果蝇合成素-1A(Syx)的同源物在神经传递中的作用已被广泛研究。然而，发育的Northern分析和原位杂交实验表明，Syx mRNA在所有发育阶段和许多组织中都有表达。我们已经分离出Syx的新突变，这些突变揭示了Syx在神经系统之外的作用。在卵巢中，Syx存在于生殖器中，但它主要定位于哺育细胞膜。生殖系中的有丝分裂重组实验表明，Syx是卵子发生所必需的，并可能参与哺育细胞的膜生物发生。在早期胚胎中，母体沉积的RNA有很大的贡献，在细胞化过程中蛋白质定位于细胞膜。在母体的贡献耗尽后，合子产生的Syx帮助胚胎发育后期发生的分泌事件，如角质层沉积和神经递质释放。然而，在幼虫的成像盘中也需要Syx，因为某些亚形突变组合显示出粗糙的眼睛和表明细胞死亡的翼口缺陷。此外，缺乏Syx的重组克隆会导致发育中眼睛的细胞死亡。我们认为，与其在角质层分泌和神经递质释放中的作用类似，Syx可能在整个果蝇发育过程中介导膜组装事件。
The role of the Drosophila homologue of syntaxin-1A (syx) in neurotransmission has been extensively studied. However, developmental Northern analyses and in situ hybridization experiments show that SYX mRNA is expressed during all stages and in many tissues. We have isolated new mutations in syx that reveal roles for syx outside the nervous system. In the ovary, SYX is present in the germarium, but it is predominantly localized to nurse cell membranes. Mitotic recombination experiments in the germline show SYX is essential for oogenesis and may participate in membrane biogenesis in the nurse cells. In the early embryo, a large contribution of maternally deposited RNA is present, and the protein is localized at cell membranes during cellularization. After the maternal contribution is depleted, zygotically produced SYX assists secretion events occurring late in embryogenesis, such as cuticle deposition and neurotransmitter release. However, SYX is also required in larval imaginal discs, as certain hypomorphic mutant combinations exhibit rough eyes and wing notch defects indicative of cell death. Furthermore, recombinant clones that lack syx cause cell lethality in the developing eye. We propose that, similar to its roles in cuticle secretion and neurotransmitter release, SYX may mediate membrane assembly events throughout Drosophila development.
影响因子：
4.6
作者：
Hugo J. Bellen;D. D'evelyn;M. Harvey;Stephen J. Elledge
通讯作者：
Hugo J. Bellen;D. D'evelyn;M. Harvey;Stephen J. Elledge
研究果蝇胚胎发生中原肠胚形成之前的五个有丝分裂周期期间的核和细胞质行为。
影响因子：
4
作者：
Foe,VE;Alberts,BM
通讯作者：
Alberts,BM