Cutaneous nerve terminal degeneration in painful mononeuropathy
Cutaneous nerve terminal degeneration in painful mononeuropathy
复制标题
DOI：
10.1006/exnr.2001.7704
复制
发表时间：
2001-08-01
影响因子：
5.3
通讯作者：
Hsieh, ST
中科院分区：
文献类型：
--
作者：
Lin, YW;Tseng, TJ;Hsieh, ST
伤害性神经支配皮肤并在神经性疼痛的产生中起重要作用。然而，它仍然是难以捉摸的伤害性神经末梢是否以及如何在神经病理性疼痛条件下退化。为了解决这个问题，我们研究了疼痛性单神经病模型，慢性压迫性损伤（CCI）的皮肤神经支配。大鼠的后爪进行免疫细胞化学染色与泛轴突标记，蛋白基因产物9.5（PGP 9.5）。CCI后2天内，大鼠表现出热痛觉过敏，并有部分耗尽的表皮神经。CCI后表皮神经减少的程度是可变的，表皮神经密度为3.65 +/- 1.97纤维/mm（与对照侧的15.39 +/- 1.58纤维/mm相比，P < 0.02）。CCI患者皮肤表皮中PGP 9.5（+）朗格汉斯细胞轻度增加（10.19 +/- 1.99 vs 7.75 +/- 1.36 cells/mm，P < 0.05）。在通过坐骨神经的紧密结扎而失神经支配的皮肤中，表皮神经完全耗尽（0纤维/mm对对照侧的12.26 +/- 1.44纤维/mm，P < 0.001）。坐骨神经紧密结扎的动物表现出热麻醉。这些结果表明，表皮部分失神经支配的CCI，和部分损伤的神经与神经病理性疼痛的发展。(C)北京：科学出版社.
Nociceptive nerves innervate the skin and play an important role in the generation of neuropathic pain. However, it remains elusive whether and how nociceptive nerve terminals degenerate in neuropathic pain conditions. To address this issue, we investigated cutaneous innervation in a model of painful mononeuropathy, the chronic constriction injury (CCI). The hind paws of rats were immunocytochemically stained with a pan-axonal marker, protein gene product 9.5 (PGP 9.5). Within 2 days after CCI, rats exhibited thermal hyperalgesia, and there was a partial depletion of epidermal nerves. The extent of reduction in epidermal nerves after CCI was variable with an epidermal nerve density of 3.65 +/- 1.97 fibers/mm (compared to 15.39 +/- 1.58 fibers/mm on the control side, P < 0.02). There was a mild but concomitant increase in PGP 9.5 (+) Langerhans cells in the epidermis of the skin with CCI (10.19 +/- 1.99 vs 7.75 +/- 1.36 cells/mm, P < 0.05). In the skin denervated by tight ligation of the sciatic nerve, epidermal nerves were completely depleted (0 fibers/mm. vs 12.26 +/- 1.44 fibers/mm on the control side, P < 0.001). Animals with tight ligation of the sciatic nerve exhibited thermal anesthesia. These findings suggest that the epidermis is partially denervated in CCI, and that a partial injury of nerves is correlated with the development of neuropathic pain. (C) 2001 Academic Press.