Barrel pattern formation requires serotonin uptake by thalamocortical afferents, and not vesicular monoamine release
Barrel pattern formation requires serotonin uptake by thalamocortical afferents, and not vesicular monoamine release
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DOI：
10.1523/jneurosci.21-17-06862.2001
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发表时间：
2001-09-01
影响因子：
5.3
通讯作者：
Keller, F
中科院分区：
文献类型：
--
作者：
Persico, AM;Mengual, E;Keller, F
在新生啮齿动物中，支配桶状皮质的丘脑皮质神经元通过表达质膜5-羟色胺转运体（5-HTT）和囊泡单胺转运体（VMAT 2）在突触囊泡中瞬时储存5-羟色胺（5-HT）。5-HTT基因敲除（ko）小鼠揭示了几乎完全没有5-HT在大脑皮层的免疫组织化学，桶，无论是在P7和成年。定量电子显微镜显示，5-HTT KO既不影响突触的密度，也不影响IV层突触接触的长度。VMAT 2 ko小鼠，完全缺乏活动依赖性囊泡释放的单胺，包括5-HT，也显示完全缺乏5-HT在皮质，但显示基本正常桶场，尽管有时显着降低出生后的增长。瞬时5-HTT的表达，因此需要桶图案的形成，而活性依赖性囊泡5-HT的释放不是。
Thalamocortical neurons innervating the barrel cortex in neonatal rodents transiently store serotonin (5-HT) in synaptic vesicles by expressing the plasma membrane serotonin transporter (5-HTT) and the vesicular monoamine transporter (VMAT2). 5-HTT knock-out (ko) mice reveal a nearly complete absence of 5-HT in the cerebral cortex by immunohistochemistry, and of barrels, both at P7 and adulthood. Quantitative electron microscopy reveals that 5-HTT ko affects neither the density of synapses nor the length of synaptic contacts in layer IV. VMAT2 ko mice, completely lacking activity-dependent vesicular release of monoamines including 5-HT, also show a complete lack of 5-HT in the cortex but display largely normal barrel fields, despite sometimes markedly reduced postnatal growth. Transient 5-HTT expression is thus required for barrel pattern formation, whereas activity-dependent vesicular 5-HT release is not.