Co-production of 1,2,4-butantriol and ethanol from lignocellulose hydrolysates
Co-production of 1,2,4-butantriol and ethanol from lignocellulose hydrolysates
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由木质纤维素水解产物联产1,2,4-丁三醇和乙醇
DOI：
10.1016/j.biortech.2019.03.057
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发表时间：
2019
影响因子：
11.4
通讯作者：
Zhuge Bin
中科院分区：
文献类型：
--
作者：
Zhao Meilin;Shi Dingchang;Lu Xinyao;Zong Hong;Zhuge Bin
为实现甘蔗渣水解液微生物发酵生产1，2，4-丁三醇（BT），并实现BT与乙醇的联产，利用产甘油假丝酵母（Candida glycerinogenesUG 21）降低水解液中高浓度葡萄糖和糠醛对BT产生菌生长的影响，并利用葡萄糖发酵生产54.1 g/L乙醇。乙醇回收后，得到含木糖的酒糟并用于BT生产。1.3 g/L BT。通过rrR基因的缺失和工艺优化，BT效价达到4.9g/L。同时，通过两段发酵法实现了甘蔗渣的高效利用，联产BT和乙醇。该研究为甘蔗渣生产BT提供了一种新的策略，并展示了充分利用甘蔗渣水解物联产高附加值产品的潜力。
The aim of this work was to realize 1,2,4-butantriol (BT) production from sugarcane bagasse hydrolysates by microbial fermentation, and obtain co-production of BT and ethanol.Candida glycerinogenesUG21 was utilized to reduce the effect of osmolality resulting from high glucose concentration and furfural in hydrolysates on cell growth of BT-producing strains, and produced 54.1 g/L ethanol from glucose. After ethanol recovering, xylose containing stillage was obtained and used for BT production. 1.3 g/L BT was generated by a BT-producing strain. By the deletion of thecrrgene and process optimization, BT titer reached 4.9 g/L. Meanwhile, the efficient utilization of sugarcane bagasse was achieved by a two-stage fermentation for co-production of BT and ethanol. This study provided a novel strategy for BT production from sugarcane bagasse, and demonstrated the potential for making full use of sugarcane bagasse hydrolysates to co-production value-added products.