Identification and evaluation of hub mRNAs and long non-coding RNAs in neutrophils during sepsis
Identification and evaluation of hub mRNAs and long non-coding RNAs in neutrophils during sepsis
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DOI：
10.1007/s00011-020-01323-3
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发表时间：
2020-03-01
影响因子：
6.7
通讯作者：
Sun, Bingwei
中科院分区：
文献类型：
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作者：
Huang, Jiamin;Sun, Ran;Sun, Bingwei
目的揭示中性粒细胞对脓毒症的系统性反应，并研究脓毒症中的核心lncrna。材料和方法采用雄性C57 BL/6小鼠股骨和胫骨的中性粒细胞。分别用1 μ g/mL脂多糖（LPS）处理中性粒细胞0、0.5、1、4 h，进行芯片检测。另外，用盲肠结扎和穿孔模拟脓毒症。在这里，我们使用不同的生物信息学分析，包括差异表达分析、加权基因共表达网络分析（WGCNA）和基因调控网络分析，来分析中性粒细胞对败血症的全身反应。结果共鉴定出9个模块，并在每个模块中发现hub lncrna。蓝色和粉色模块与脓毒症的炎症状态密切相关。一些枢纽lncrna （NONMMUT005259， KnowTID_00004196和NR_003507）可能具有与脓毒症中的炎症状态相关的功能。基于一种新的生物学方法，我们的研究结果揭示了中性粒细胞对脓毒症的全身水平反应，并鉴定了几种对脓毒症具有潜在调节作用的枢纽lncrna。
Objective To reveal the systematic response of neutrophils to sepsis and to study the hub lncRNAs in sepsis. Materials and methods Neutrophils taken from the femur and tibia of male C57 BL/6 mice were used in this study. And neutrophils were treated for 0 h, 0.5 h, 1 h, and 4 h with or without 1 mu g/mL lipopolysaccharide (LPS) for further chip detection. In addition, cecal ligation and perforation were used to simulate sepsis. Here, we used different bioinformatics analyses, including differential expression analysis, weighted gene co-expression network analysis (WGCNA), and gene regulatory network analysis, to analyze the systemic response of neutrophils to sepsis. Results We identified nine modules and found hub lncRNAs in each module. The blue and pink modules were closely related to the inflammatory state of sepsis. Some hub lncRNAs (NONMMUT005259, KnowTID_00004196, and NR_003507) may have functions related to the inflammatory state in sepsis. Conclusions Based on a new biological approach, our research results revealed the systemic-level response of neutrophils to sepsis and identified several hub lncRNAs with potential regulatory effects on this condition.