Sequence type ST405 Escherichia coli isolate producing QepA1, CTX-M-15, and RmtB from Detroit, Michigan.
Sequence type ST405 Escherichia coli isolate producing QepA1, CTX-M-15, and RmtB from Detroit, Michigan.
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来自密歇根州底特律的序列类型 ST405 大肠杆菌分离株，产生 QepA1、CTX-M-15 和 RmtB。
DOI：
10.1128/aac.00652-11
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发表时间：
2011
影响因子：
4.9
通讯作者：
Doi,Yohei
中科院分区：
文献类型：
--
作者：
Tian,Guo-Bao;Rivera,JesabelI;Park,YoonSoo;Johnson,LauraE;Hingwe,Ameet;Adams-Haduch,JenniferM;Doi,Yohei
质粒介导的氟喹诺酮类药物耐药决定因素在世界范围内越来越多地在肠杆菌科的各种分离株中被发现(9)。其中，QepA属于主要助剂超家族（MFS）型外排泵组。它最初是在比利时和日本的大肠杆菌临床分离株中发现的（8,13）。QepA（现为QepA1）随后在人（1,5,10）和动物（6,7）源大肠杆菌分离株中检测到。QepA2是QepA1的一种双氨基酸变体，已在法国的大肠杆菌临床分离株中报道(2)。在这里，我们报告在美国大肠杆菌分离物中鉴定出QepA1。大肠杆菌M3006是从一名47岁截瘫女性患者的尿液中分离出来的，该患者于2010年1月从一家养老院入住密歇根州底特律的一家医院，治疗骶骨骨髓炎。她在就诊前已接受环丙沙星治疗10天。该分离株对头孢他啶、头孢噻肟、头孢吡肟、环丙沙星和阿米卡星高度耐药，mic均为32 g/ml，对所有4种碳青霉烯类药物（厄他培南、亚胺培南、美罗培南和多利培南）均敏感。PCR分析鉴定了多种耐药基因，包括内酰胺酶基因blaTEM、blaSHV和blaCTX-M；16S rRNA甲基化酶基因armA， rmtB和
Plasmid-mediated ffuoroquinolone resistance determinants are increasingly identified worldwide among various isolates of Enterobacteriaceae (9). Among them, QepA belongs to the major facilitator superfamily (MFS)-type group of efffux pumps. It was initially identified in Escherichia coli clinical isolates from Belgium and Japan (8, 13). QepA (now QepA1) has subsequently been detected in E. coli isolates of human (1, 5, 10) and animal (6, 7) origins. QepA2, a two-amino-acid variant of QepA1, has been reported from an E. coli clinical isolate in France (2).Here we report identification of QepA1 in an E. coli isolate from the United States. E. coli M3006 was isolated from the urine of a 47-year-old female patient with paraplegia, who was admitted from a nursing home to a hospital in Detroit, MI, for treatment of sacral osteomyelitis in January 2010. She had received ciproffoxacin for 10 days just prior to her presentation. The isolate was highly resistant to ceftazidime, cefotaxime, cefepime, ciproffoxacin, and amikacin, all with MICs of 32 g/ml, and susceptible to all four carbapenems (ertapenem, imipenem, meropenem, and doripenem). PCR analysis was performed to identify various resistance genes, including-lactamase genes blaTEM, blaSHV, and blaCTX-M; 16S rRNA methylase genes armA, rmtB, and