Protection of Siganus oramin, rabbitfish, from heavy metal toxicity by the selenium-enriched seaweed Gracilaria lemaneiformis.
Protection of Siganus oramin, rabbitfish, from heavy metal toxicity by the selenium-enriched seaweed Gracilaria lemaneiformis.
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DOI：
10.1016/j.ecoenv.2020.111183
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发表时间：
2020-09
影响因子：
6.8
通讯作者：
Hongtian Luo;Yufeng Yang;Qing Wang;Yongjie Wu;Zhili He;Wenbo Yu
中科院分区：
文献类型：
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作者：
Hongtian Luo;Yufeng Yang;Qing Wang;Yongjie Wu;Zhili He;Wenbo Yu
海藻是海洋生态系统中本质上重要的实体。它不仅被水生动物食用，而且还改善了海水养殖的环境质量。海藻也是人类饮食的一部分。本研究的目的是评估富含硒 (Se) 的龙须草对重金属的拮抗作用，特别是膳食中富含硒的龙须草预防兔鱼 (Siganus oramin) 重金属毒性的潜力。生长速率、重金属（Se、Cd、Pb、Cu、Zn 和 Cr）浓度、丙二醛 (MDA)、金属硫蛋白 (MT) 以及抗氧化剂、谷胱甘肽过氧化物酶 (GPX)、过氧化氢酶 (CAT) 和超氧化物歧化酶 (SOD) 的活性均进行了评估。结果表明，富集3 d后，250 mg L−1Se富集龙须草的总有机和无机硒浓度显着高于50和10 mg L−1处理。 250 mg L−1 处理中江蓠的平均总硒浓度为 42.5 μg g−1，50 mg L−1 处理中为 13.5 μg g−1，10 mg L−1 处理中为 2.5 μg g−1。富硒江蓠中有机硒占总硒的80%~82%。饲喂富硒江蓠的兔鱼硒浓度显着高于对照。此外，Se 还能增加 Cu 和 Zn 的吸收，增强 MT 的生成，提高 GPX、CAT 和 SOD 抗氧化活性，降低 MDA 浓度和脂质过氧化水平，所有这些都与 Cd、Pb 和 Cr 拮抗。富硒江蓠水性镉、铅和铬引起的毒性作用是通过酶促和非酶促抗氧化机制在兔鱼中发生的。硒对兔鱼体内的锌、铜有协同作用。对于50 mg L−1Se富集龙须草处理，兔鱼中Se、Cu、Zn和拮抗Cd、Pb、Cr以及抗氧化酶CAT、SOD、GPX活性和MT浓度均高于250 mg L−1和10 mg L−1Se富集龙须草处理。 50 mg L−1Se处理江蓠被认为是促进兔鱼生长的最佳浓度。因此，结果表明，富硒江蓠具有拮抗重金属毒性的作用，是提高养殖动物食用安全性的适宜硒补充剂。
Seaweed is an inherently important entity in marine ecosystems. It is not only consumed by aquatic animals but also improves environmental quality in the mariculture. Seaweed is also part of the diet of human beings. The purpose of the present study was to evaluate the antagonism of selenium (Se)-enrichedGracilaria lemaneiformisagainst heavy metals, specifically, the potential of dietary Se-enrichedGracilariato protect against heavy metal toxicity in rabbitfish (Siganus oramin). Growth rate, heavy metal (Se, Cd, Pb, Cu, Zn and Cr) concentrations, malondialdehyde (MDA), metallothionein (MT), and the activity of the antioxidants, glutathione peroxidase (GPX), catalase (CAT) and superoxide dismutase (SOD) were all assessed. The results showed that the total organic and inorganic Se concentration for the 250 mg L−1Se-enrichedGracilariawas significantly higher than those of the 50 and 10 mg L−1treatments after 3 days of enrichment. The mean total Se concentrations inGracilariawere 42.5 μg g−1in the 250 mg L−1treatment, 13.5 μg g−1in the 50 mg L−1treatment and 2.5 μg g−1in the 10 mg L−1treatment, respectively. Organic Se accounts for 80–82% of total Se in Se-enrichedGracilaria. The Se concentration of rabbitfish fed Se-enrichedGracilariawas significantly higher than control. Furthermore, Se increased Cu and Zn absorption, and enhanced MT generation, and improved GPX, CAT, and SOD antioxidant activity, and decreased MDA concentrations and lipid peroxidation levels, all antagonistic to Cd, Pb and Cr. The effects of Se-enrichedGracilariaon waterborne Cd, Pb and Cr-induced toxicity occurred via both enzymatic and non-enzymatic antioxidative mechanisms in rabbitfish. Selenium had synergistic effects on Zn and Cu in rabbitfish. For the 50 mg L−1Se-enrichedGracilariatreatment, the Se, Cu, Zn, and antagonistic Cd, Pb, Cr, and the antioxidant enzymes CAT, SOD, GPX activities, and MT concentrations in rabbitfish were higher than that with the 250 mg L−1and 10 mg L−1Se-enrichedGracilariatreatments. The 50 mg L−1Se treatment ofGracilariawas deemed to be the optimum concentration to promote growth of rabbitfish. Therefore, the obtained results suggest Se-enrichedGracilariacan antagonize heavy metal toxicity, and is an advisable Se supplement to improve the edible safety of cultured animals.