REVERSIBLE PHASE VARIATION OF EXPRESSION OF NEISSERIA-MENINGITIDIS CLASS-5 OUTER-MEMBRANE PROTEINS AND THEIR RELATIONSHIP TO GONOCOCCAL PROTEINS-II
REVERSIBLE PHASE VARIATION OF EXPRESSION OF NEISSERIA-MENINGITIDIS CLASS-5 OUTER-MEMBRANE PROTEINS AND THEIR RELATIONSHIP TO GONOCOCCAL PROTEINS-II
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DOI：
10.1128/iai.56.2.380-386.1988
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发表时间：
1988-02-01
影响因子：
3.1
通讯作者：
CANNON, JG
中科院分区：
文献类型：
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作者：
KAWULA, TH;AHO, EL;CANNON, JG
脑膜炎奈瑟菌 5 类蛋白是主要的外膜蛋白，与淋病奈瑟菌的蛋白 II (P.II) 具有许多相同的特性。我们生成了两种杀菌单克隆抗体，每种抗体都与脑膜炎奈瑟氏球菌 FAM18 产生的两种已确定的 5 类蛋白之一特异性结合。单克隆抗体还与有限数量的其他脑膜炎球菌菌株的 5 类蛋白结合。利用单克隆抗体的杀菌活性，我们证明了两种 5 类蛋白的表达在体外都发生可逆的相变。纯化的 5 类蛋白的 N 端氨基酸序列与淋球菌 P.II 蛋白的 N 端氨基酸序列惊人相似。使用克隆的 5 类基因，我们在脑膜炎奈瑟菌 FAM18 中鉴定了三个潜在的 5 类基因位点。这些5类序列还与淋球菌P.II基因序列具有同源性，并含有被认为在淋球菌P.II表达调节中重要的CTCTT重复序列。
Neisseria meningitidis class 5 proteins are major outer membrane proteins that share many properties with the proteins II (P.II) of Neisseria gonorrhoeae. We generated two bactericidal monoclonal antibodies, each of which bound specifically to one of the two indentified class 5 proteins produced by N. meningitidis FAM18. The monoclonal antibodies also bound to class 5 proteins of a limited number of other meningococcal strains. Using the bactericidal activity of the monoclonal antibodies, we demonstrated that expression of both class 5 proteins was subject to reversible phase variation in vitro. The N-terminal amino acid sequence of a purified class 5 protein revealed striking similarity to the N-terminal amino acid sequence of gonococcal P.II proteins. Using a cloned class 5 gene, we identified three potential class 5 gene loci in N. meningitidis FAM18. These class 5 sequences also had homology with gonococcal P.II gene sequences and contained the CTCTT repeat sequence believed to be important in the regulation of gonococcal P.II expression.