Continuous Colorimetric Assay for Acetylcholinesterase and Inhibitor Screening with Gold Nanoparticles
Continuous Colorimetric Assay for Acetylcholinesterase and Inhibitor Screening with Gold Nanoparticles
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DOI：
10.1021/la803870v
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发表时间：
2009-02-17
期刊：
影响因子：
3.9
通讯作者：
Zhu, Daoben
中科院分区：
文献类型：
--
作者：
Wang, Ming;Gu, Xinggui;Zhu, Daoben
本文报道了一种新的比色法测定乙酰胆碱酯酶（AChE）活性及其抑制剂的筛选方法，该方法利用了以下事实：（1）金纳米颗粒（Au-NPs）的聚集导致等离子体吸收的红移，这是由于颗粒间的等离子体相互作用;（2）AChE可以催化乙酰硫代胆碱水解为硫代胆碱，从而诱导Au-NPs的聚集。用这种简便的方法，AChE的活性与浓度低至0.6 mU/mL可以测定。此外，该测定方法也可用于筛选AChE抑制剂。该方法简单易行，可用于高通量筛选乙酰胆碱酯酶抑制剂及相关药物的开发。
We report herein a new colorimetric assay method for acetylcholinesterase (AChE) activity and its inhibitor screening by making use of the following facts: (1) the aggregation of gold nanoparticles (Au-NPs) results in the red-shift of the plasmon absorption due to interparticle plasmon interactions and (2) AChE can catalyze the hydrolysis of acetylthiocholine into thiocholine which can induce the aggregation of Au-NPs. With this convenient method, the activity of AChE with a concentration as low as 0.6 mU/mL can be assayed. Moreover, this assay method is also useful for screening inhibitors of AChE. Given its simplicity and easy-operation, this method may extend to high-throughput screening of AChE inhibitors and relevant drug discovery.