The effects of ovariectomy, estrogen replacement, and neuraminidase treatment on the properties of the adenohypophysial glycoprotein hormones of the Rhesus monkey.
The effects of ovariectomy, estrogen replacement, and neuraminidase treatment on the properties of the adenohypophysial glycoprotein hormones of the Rhesus monkey.
复制标题
卵巢切除、雌激素替代和神经氨酸酶治疗对恒河猴腺垂体糖蛋白激素特性的影响。
作者：
W. Peckham;Ernst Knobil
对来自完整和卵巢切除猴的垂体提取物的比较显示，与来自完整雌性的LH相比，来自卵巢切除动物的LH的平均表观分子大小更大，并且从试验大鼠的循环中消失的速度更慢。相反，卵巢切除术对TSH的表观分子大小没有影响，也没有影响注射到实验大鼠后的消失率。卵巢切除术对LH和FSH分子大小的影响通过长期给予雌二醇来实现。完整和卵巢切除猴促性腺激素的表观分子大小的差异，减少或取消与酶神经氨酸酶消化。这些结果表明，卵巢切除术后促性腺激素的质的变化代表了对雌激素去除的特异性反应，并且与这种现象涉及促性腺激素唾液酸含量变化的观点一致。
A comparison of pituitary extracts from intact and ovariectomized monkeys revealed that, like FSH, LH from ovariectomized animals is larger in average apparent molecular size and disappears from the circulation of test rats less rapidly than does LH from intact females. In contrast, ovariectomy had no effect on the apparent molecular size of TSH nor on its disappearance rate following injection into test rats. Reversal of the effect of ovariectomy on the molecular size of both LH and FSH was achieved by the chronic administration of estradiol. The differences in apparent molecular size of gonadotropins from intact and ovariectomized monkeys were either reduced or abolished by digestion with the enzyme neuraminidase. These findings suggest that the qualitative changes in the gonadotropins consequent to ovariectomy represent a specific response to the removal of estrogen and are consonant with the view that this phenomenon involves changes in the sialic acid content of the gonadotropins.