Biocompatible and biodegradable ultrafine fibrillar scaffold materials for tissue engineering by facile grafting of L-lactide onto chitosan
Biocompatible and biodegradable ultrafine fibrillar scaffold materials for tissue engineering by facile grafting of L-lactide onto chitosan
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DOI：
10.1021/bm800158c
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发表时间：
2008-07-01
影响因子：
6.2
通讯作者：
Subramanian, Anuradha
中科院分区：
文献类型：
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作者：
Skotak, Maciej;Leonov, Alexei P.;Subramanian, Anuradha
采用“一锅法”接枝L-丙交酯低聚物，以良好的收率制备了壳聚糖衍生物；通过开环聚合。侧链主要连接至位于葡糖胺环的碳 3 和碳 6 上的羟基，而胺基保持非官能化。通过增加 L-丙交酯与壳聚糖的比例，可以控制侧链长度。这允许控制组织工程支架材料的生物降解率和亲水性。这种通用合成路线使功能化壳聚糖可溶于多种有机溶剂，从而促进通过静电纺丝形成超细纤维。使用成纤维细胞（L929细胞系）对电纺L-丙交酯改性壳聚糖纤维进行的细胞毒性测试表明，本研究中研究的具有最高L-丙交酯摩尔比（1:24）的样本是用于组织工程目的最有前途的材料，而稳定性较差的配方仍可能在药物输送载体中得到应用。
A chitosan derivative was prepared with good yields using a "one pot" approach by grafting L-lactide oligomers; via ring opening polymerization. Side chains are primarily attached to hydroxyl groups located on carbons 3 and 6 of the glucosamine ring, while the amine group remains nonfunctionalized. By increasing the L-lactide to chitosan ratio, side chain length is controlled. This allows the manipulation of the biodegradation rate and hydrophilicity of the tissue engineering scaffold material. This general synthetic route renders functionalized chitosan soluble in a broad range of organic solvents, facilitating formation of ultrafine fibers via electrospinning. Cytotoxicity tests using fibroblasts (L929 cell line) performed on electrospun L-lactide modified chitosan fibers showed that the specimen with the highest molar ratio Of L-lactide (1:24) investigated in this study is the most promising material for tissue engineering purposes, while less stable formulations might still find application in drug delivery vehicles.