A ras-related small GTP-binding protein, RabE1c, regulates stomatal movements and drought stress responses by mediating the interaction with ABA receptors
A ras-related small GTP-binding protein, RabE1c, regulates stomatal movements and drought stress responses by mediating the interaction with ABA receptors
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Ras 相关的小 GTP 结合蛋白 RabE1c 通过介导与 ABA 受体的相互作用来调节气孔运动和干旱胁迫反应。
DOI：
10.1016/j.plantsci.2021.110858
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发表时间：
2021-02-27
期刊：
影响因子：
5.2
通讯作者：
Wang, Mei
中科院分区：
文献类型：
--
作者：
Chen, Donghua;He, Lilong;Wang, Mei
干旱是全球农作物产量的主要制约因素。植物对这种胁迫的反应集中在细胞膜的行为上，在细胞膜上发生脱落酸（ABA）信号的转导。研究表明，ras相关的小gtp结合蛋白RabE1c能够与拟南芥质膜中的ABA受体结合，从而正向调节ABA信号。RabE1c受干旱胁迫高度诱导，在保护细胞中大量表达。在失去功能的rabe1c突变体中，气孔关闭和全株干旱胁迫反应对ABA处理的敏感性均降低，表明rabe1c参与了通过气孔控制蒸发水损失的过程。RabE1c损伤？s的功能抑制了ABA受体PYL4的积累。RabE1c在拟南芥中的过表达增强了植株的抗氧化能力。该基因在大白菜（Brassica rapassp）中的组成性表达也获得了类似的表型效应。学报)。主要结论是RabE1c促进PYL4的降解，这表明一种可能的遗传策略可以使作物更好地抵御干旱胁迫。
Drought represents a leading constraint over crop productivity worldwide. The plant response to this stress is centered on the behavior of the cell membrane, where the transduction of abscisic acid (ABA) signaling occurs. Here, the Ras-related small GTP-binding protein RabE1c has been shown able to bind to an ABA receptor in the Arabidopsis thaliana plasma membrane, thereby positively regulating ABA signaling. RabE1c is highly induced by drought stress and expressed abundantly in guard cells. In the loss-of-function rabe1c mutant, both stomatal closure and the whole plant drought stress response showed a reduced sensitivity to ABA treatment, demonstrating that RabE1c is involved in the control over transpirative water loss through the stomata. Impairment of RabE1c?s function suppressed the accumulation of the ABA receptor PYL4. The over-expression of RabE1c in A. thaliana enhanced the plants? ability to tolerate drought, and a similar phenotypic effect was achieved by constitutively expressing the gene in Chinese cabbage (Brassica rapassp. pekinensis). The leading conclusion was that RabE1c promotes the degradation of PYL4, suggesting a possible genetic strategy to engineer crop plants to better withstand drought stress.