Two new pseudopod morphologies displayed by the human hematopoietic KG1a progenitor cell line and by primary human CD34+ cells
Two new pseudopod morphologies displayed by the human hematopoietic KG1a progenitor cell line and by primary human CD34+ cells
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DOI：
10.1182/blood.v92.10.3616.422k19_3616_3623
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发表时间：
1998-11-15
期刊：
影响因子：
20.3
通讯作者：
Palsson, BO
中科院分区：
文献类型：
--
作者：
Francis, K;Ramakrishna, R;Palsson, BO
一种以CD34表面抗原高表达为特征的原始人造血髓祖细胞系KG1a被观察到延伸长而薄的假足。一旦伸展，这些假足可能呈现两种新描述的形态之一。腱足或巨足。腱足很细，呈线状。它们粘附在底物上，可以多次分叉，并且经常出现在间隔超过300 μ m的细胞膜上。大足要厚得多，观察到它可以延伸到离细胞330多亩的地方。磁足动物是灵活的，可以表现出快速的动态运动，在几秒钟内伸展或收缩。在缩回过程中，延伸的材料通常聚集成位于豆荚上的球泡。这两种形态都可以粘附在涂有纤维连接蛋白的底物上。胶原蛋白，层粘连蛋白和塑料。CD34和CD44抗原也存在于这些足表面。来自胎儿肝脏、脐带血、成人骨髓和动员的外周血的原代人CD34（+）细胞也延伸这些足部。这些伪足的形态表明它们可能在骨髓移植后的细胞迁移和归巢过程中发挥感觉和机械作用。(C) 1998年由美国血液病学会出版。
A primitive human hematopoietic myeloid progenitor cell line, KG1a, characterized by high expression of the CD34 surface antigen has been observed to extend long, thin pseudopodia. Once extended, these pseudopods may take on one of two newly described morphologies. tenupodia or magnupodia. Tenupodia are very thin and form in linear segments. They adhere to the substrate, can bifurcate multiple times, and often appear to connect the membranes of cells more than 300 mu m apart. Magnupodia are much thicker and have been observed to extend more than 330 mu m away from the cell. Magnupods are flexible and can exhibit rapid dynamic motion, extending or retracting in a few seconds. During retraction, the extended material often pools into a bulb located on the pod. Both morphologies can adhere to substrates coated with fibronectin. collagen IV, and laminin as well as plastic. The CD34 and CD44 antigens are also present on the surface of these podia. Primary human CD34(+) cells from fetal liver, umbilical cord blood, adult bone marrow, and mobilized peripheral blood extend these podia as well. The morphology that these pseudopods exhibit suggest that they may play both sensory and mechanical roles during cell migration and homing after bone marrow transplantation. (C) 1998 by The American Society of Hematology..