Opposing actions of septins and Sticky on Anillin promote the transition from contractile to midbody ring.
Opposing actions of septins and Sticky on Anillin promote the transition from contractile to midbody ring.
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DOI：
10.1083/jcb.201305053
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发表时间：
2013-11-11
期刊：
影响因子：
--
通讯作者：
Hickson GR
中科院分区：
文献类型：
--
作者：
El Amine N;Kechad A;Jananji S;Hickson GR
依赖于Septin的膜相关苯胺素的去除和粘性依赖的苯胺素保留对于收缩环的稳定性和闭合以及中体环的形成是必需的。在胞质分裂过程中，肌动球蛋白收缩环(CR)的闭合与中体环(MR)的形成通过鲜为人知的机制相耦合。利用延时显微镜对果蝇黑腹果蝇S2细胞的研究表明，从CR到MR的转变是通过一个以前未知的成熟过程进行的，这个过程需要相反的机制来去除和保留支架蛋白ANLIN。Septin细胞骨架作用于ANILIN的C末端，通过内化、挤出和脱落，局部修剪晚期CR/新生MR上多余的膜，而柠檬酸激酶Sticky作用于ANILIN的N端，将其保留在成熟的MR上。我们的发现强调了CR和MR的相关性，并提示膜去除与CR拆卸是相协调的。
Septin-dependent removal of membrane-associated Anillin and Sticky-dependent retention of Anillin are required for contractile ring stability and closure and for midbody ring formation. During cytokinesis, closure of the actomyosin contractile ring (CR) is coupled to the formation of a midbody ring (MR), through poorly understood mechanisms. Using time-lapse microscopy of Drosophila melanogaster S2 cells, we show that the transition from the CR to the MR proceeds via a previously uncharacterized maturation process that requires opposing mechanisms of removal and retention of the scaffold protein Anillin. The septin cytoskeleton acts on the C terminus of Anillin to locally trim away excess membrane from the late CR/nascent MR via internalization, extrusion, and shedding, whereas the citron kinase Sticky acts on the N terminus of Anillin to retain it at the mature MR. Simultaneous depletion of septins and Sticky not only disrupted MR formation but also caused earlier CR oscillations, uncovering redundant mechanisms of CR stability that can partly explain the essential role of Anillin in this process. Our findings highlight the relatedness of the CR and MR and suggest that membrane removal is coordinated with CR disassembly.
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