SCRAPIE INOCULATION OF MICE - LIGHT AND ELECTRON-MICROSCOPY OF THE SUPERIOR COLLICULI
SCRAPIE INOCULATION OF MICE - LIGHT AND ELECTRON-MICROSCOPY OF THE SUPERIOR COLLICULI
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DOI：
10.1007/bf00310139
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发表时间：
1991-01-01
影响因子：
12.7
通讯作者：
FRASER, H
中科院分区：
文献类型：
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作者：
JEFFREY, M;SCOTT, JR;FRASER, H
眼内接种痒病ME7株小鼠的上丘超微结构检查显示，感染早期出现空泡化。在单眼接种痒病后85-260天对大脑进行检查。临床发病潜伏期平均为302天。空泡化最初见于对侧上丘，随后见于同侧小丘。在编码试验中，从218天开始可见光镜下的空泡形成，但从85天开始的超微结构检查显示，稀疏的空泡不一致地存在于同侧和对侧丘中；从第190天开始可见频繁的液泡。痒病诱导的液泡与对照组织中的液泡是有区别的，液泡的存在是通过定位的存在，或者是通过限制性的双膜（最内层片的突出或增殖）来实现的。神经核周、髓鞘纤维、树突和轴突突触前终末可见空泡形成。髓鞘内可见髓鞘纤维液泡，可能在少突胶质细胞质内舌也可见。螺旋状的膜结构也可见。在两只感染痒病的小鼠中发现了直径为40纳米的管泡颗粒。提示一些痒病液泡的产生是由于异常的膜与细胞器结合，可能是线粒体，在神经核周和神经突中，也可能是在少突胶质细胞质和髓鞘中。
Ultrastructural examination of the superior colliculi of mice intraocularly inoculated with the ME7 strain of scrapie showed vacuolation early in the course of infection. Brains were examined between 85-260 days after monocular inoculation with scrapie. The mean incubation period for the development of clinical disease was 302 days. Vacuolation was seen initially in the contralateral superior colliculus and subsequently in the ipsilateral colliculus. In coded trials light microscopical vacuolation was seen from 218 days but ultrastructural examination showed that sparse vacuoles were inconsistently present in either or both of the ipsilateral and contralateral colliculi from 85 days; frequent vacuoles were seen from 190 days. Scrapie-induced vacuoles were differentiated from vacuoles present in control tissue by the presence of loculation or by a limiting double membrane which showed protrusion or proliferation of the innermost lamella. Vacuolation was seen in neuronal perikarya, myelinated fibres, dendrites and axonal presynaptic terminals. Vacuoles of myelinated fibres were observed within myelin and possibly also in the inner tongue of oligodendroglial cytoplasm. Whorled membrane configurations were also seen. Tubulovesicular particles, 40 nm in diameter, were recognised in two scrapie-infected mice. It is suggested that some scrapie vacuoles arise as a result of incorporation of abnormal membrane into organelles, possibly mitochondria, in neuronal perikarya and neurites and probably also within oligodendroglial cytoplasm and myelin.