A Fluorescently Labeled Dendronized Polymer-Enzyme Conjugate Carrying Multiple Copies of Two Different Types of Active Enzymes
A Fluorescently Labeled Dendronized Polymer-Enzyme Conjugate Carrying Multiple Copies of Two Different Types of Active Enzymes
复制标题
DOI：
10.1021/ja304837f
复制
发表时间：
2012-07-18
影响因子：
15
通讯作者：
Walde, Peter
中科院分区：
文献类型：
--
作者：
Grotzky, Andrea;Nauser, Thomas;Walde, Peter
合成了树枝状聚合物、两种不同类型的酶(超氧化物歧化酶和辣根过氧化物酶)和一种荧光染料(荧光素)的杂化结构。因此，单个聚合链携带这两种酶和荧光素的多个副本。整个吸附化学是基于UV/VIS可定量的双芳基腙键的形成，该键允许直接定量结合的分子：60个超氧化物歧化酶、120个辣根过氧化物酶和20个荧光素分子，这些分子位于2000个重复单元的平均聚合物链上。为了获得其他酶的比例，对实验条件进行了相应的改变。此外，这两种酶都保持了完全的活性，并催化了两步级联反应。
A hybrid structure of a synthetic dendronized polymer, two different types of enzymes (superoxide dismutase and horseradish peroxidase), and a fluorescent dye (fluorescein) was synthesized. Thereby, a single polymer chain carried multiple copies of the two enzymes and the fluorescein. The entire attachment chemistry is based on UV/vis-quantifiable bis-aryl hydrazone bond formation that allows direct quantification of bound molecules: 60 superoxide dismutase, 120 horseradish peroxidase, and 20 fluorescein molecules on an average polymer chain of 2000 repeating units. To obtain other enzyme ratios the experimental conditions were altered accordingly. Moreover, it could be shown that both enzymes remained fully active and catalyzed a two-step cascade reaction.