MOLECULAR-CLONING OF GENES UNDER CONTROL OF THE CIRCADIAN CLOCK IN NEUROSPORA
MOLECULAR-CLONING OF GENES UNDER CONTROL OF THE CIRCADIAN CLOCK IN NEUROSPORA
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DOI：
10.1126/science.2563175
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发表时间：
1989-01-20
期刊：
影响因子：
56.9
通讯作者：
DUNLAP, JC
中科院分区：
文献类型：
--
作者：
LOROS, JJ;DENOME, SA;DUNLAP, JC
为了研究生物钟对信使RNA丰度的调节，我们用互补DNA探针筛选基因组和互补DNA文库，通过连续的减法杂交，富集与神经孢子虫清晨或傍晚培养特异性转录本互补的序列。通过这种方法只鉴定出两个早晨特异性基因。RNA印迹分析证实，这些基因转录本的丰度在时钟野生型菌株中以21.5小时为周期振荡，在长周期时钟突变菌株frq7中以29小时为周期振荡。通过使用限制性片段长度多态性进行遗传作图显示，ccg-1和ccg-2这两个基因没有连锁。这些数据提供了基因表达的时钟控制程度的观点。
To investigate the regulation of messenger RNA abundance by circadian clocks, genomic and complementary DNA libraries were screened with complementary DNA probes enriched, by means of sequential rounds of subtractive hybridization, for sequences complementary to transcripts specific to either early morning or early evening cultures of Neurospora. Only two morning-specific genes were identified through this protocol. RNA blot analysis verified that the abundance of the transcripts arising from these genes oscillates with a period of 21.5 hours in a clock wild-tpe strain and 29 hours in the long-period clock mutant strain frq7. Genetic mapping through the use of restriction fragment length polymorphisms shows the two genes, ccg-1 and ccg-2, to be unlinked. These data provide a view of the extent of clock control of gene expression.