Identification of Single Identification of Single MeloidogyneMeloidogyne Juveniles by Polymerase Juveniles by Polymerase Chain Reaction Amplification of Mitochondrial DNA Chain Reaction Amplification of Mitochondrial DNA
Identification of Single Identification of Single MeloidogyneMeloidogyne Juveniles by Polymerase Juveniles by Polymerase Chain Reaction Amplification of Mitochondrial DNA Chain Reaction Amplification of Mitochondrial DNA
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发表时间：
2019
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通讯作者：
S. T;Harris;J. L.;Sandall
中科院分区：
文献类型：
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作者：
S. T;Harris;J. L.;Sandall
采用聚合酶链反应（PCR）技术，从南方根结线虫（Meloidogyne incognita，M.哈普拉，M。javanica和M.砂壳每个反应产生约2 μ g扩增产物。用HinfI限制性消化扩增产物，可以区分这四个物种的克隆谱系。而爪哇根结线虫（Meloidogyne javanica）不能与M. hapla通过这些实验中使用的酶。为了优化鉴定的速度和质量，对各种扩增条件和线虫裂解程序进行了研究。
Polymerase chain reaction (PCR) was used to amplify a specific 1.8-kb sequence of mitochondrial DNA from single juveniles and eggs from 17 populations ofMeloidogyne incognita, M. hapla, M. javanica, and M. arenaria. Approximately 2 #g amplified product were produced per reaction. Restriction digestion of the amplified product with HinfI permitted discrimination of clonal lineages of the four species. Meloidogyne javanica, however, could not be separated from M. hapla by the enzymes used in these experiments. Various amplification conditions and nematode lysis procedures were examined in order to optimize the speed and quality of identifications.