Silicon micromachining to tissue engineer branched vascular channels for liver fabrication
Silicon micromachining to tissue engineer branched vascular channels for liver fabrication
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DOI：
10.1089/107632700320739
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发表时间：
2000-04-01
影响因子：
--
通讯作者：
Vacanti, JP
中科院分区：
文献类型：
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作者：
Kaihara, S;Borenstein, J;Vacanti, JP
迄今为止，已经出现了许多工程化新组织的方法，并且它们都依赖于来自宿主的血管化以提供永久植入和氧气和营养物质的质量转移。虽然这种方法在许多组织中是有用的，但它在厚的复杂组织中并不成功，特别是那些包括大的重要器官如肝脏、肾脏和心脏的组织。在这项研究中，我们报告了使用硅和Pyrex表面上的微加工技术来生成完整的血管系统的初步结果，这些血管系统可以在植入前与工程组织集成。使用标准的光刻技术，沟槽图案让人联想到血管和毛细血管网络的分支结构蚀刻到硅和Pyrex表面作为模板。培养肝细胞和内皮细胞，随后从这些二维模具中取出单细胞单层。两种细胞类型在这些表面上都是活的和增殖的。此外，肝细胞维持白蛋白生成。然后将提升的单层折叠成紧凑的三维组织。因此，随着在组织工程中使用微细加工技术，现在似乎可以考虑从二维模板中提升内皮细胞作为分支血管网络，其最终可以与实质组织层（例如肝细胞）组合，以形成用于植入的活血管化组织的三维构象。
To date, many approaches to engineering new tissue have emerged and they have all relied on vascularization from the host to provide permanent engraftment and mass transfer of oxygen and nutrients. Although this approach has been useful in many tissues, it has not been as successful in thick, complex tissues, particularly those comprising the large vital organs such as the liver, kidney, and heart. In this study, we report preliminary results using micromachining technologies on silicon and Pyrex surfaces to generate complete vascular systems that may be integrated with engineered tissue before implantation. Using standard photolithography techniques, trench patterns reminiscent of branched architecture of vascular and capillary networks were etched onto silicon and Pyrex surfaces to serve as templates. Hepatocytes and endothelial cells were cultured and subsequently lifted as single-cell monolayers from these two-dimensional molds. Both cell types were viable and proliferative on these surfaces. In addition, hepatocytes maintained albumin production. The lifted monolayers were then folded into compact three-dimensional tissues. Thus, with the use microfabrication technology in tissue engineering, it now seems feasible to consider lifting endothelial cells as branched vascular networks from two-dimensional templates that mag ultimately be combined with layers of parenchymal tissue, such as hepatocytes, to form three-dimensional conformations of living vascularized tissue for implantation.