Specific adsorption of a β-lactam antibiotic in vivo by an anion-exchange resin for protection of the intestinal microbiota
Specific adsorption of a β-lactam antibiotic in vivo by an anion-exchange resin for protection of the intestinal microbiota
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DOI：
10.1039/d1bm00958c
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发表时间：
2021-09-21
影响因子：
6.6
通讯作者：
Katayama, Yoshiki
中科院分区：
文献类型：
--
作者：
Li, Shunyi;Yakabe, Kyosuke;Katayama, Yoshiki
在给药过程中，从肠道排出的抗生素部分会导致共生菌的破坏，并导致生态失调和各种疾病。为了在抗生素治疗期间保护肠道微生物群，活性炭（AC）的使用最近被报道为一种吸附抗生素的方法。然而，AC对抗生素的吸附是非特异性的，也可能导致对必需生物分子的吸附。在这项工作中，我们报道了阴离子交换树脂（AER）在吸附β -内酰胺类抗生素头孢哌酮（CEF）方面比AC具有更好的特异性。由于CEF具有带负电荷的羧酸基团和共轭体系，因此AER通过静电和π - π相互作用吸附CEF。AER对肠内胆汁酸和维生素等生物分子的CEF具有特异性。AER对体外培养的大肠杆菌有保护作用。此外，口服AER降低了粪便中游离的CEF浓度，并保护肠道微生物群免受CEF诱导的生态失调。
The fraction of antibiotics that are excreted from the intestine during administration leads to disruption of commensal bacteria as well as resulting in dysbiosis and various diseases. To protect the gut microbiota during treatment with antibiotics, use of activated carbon (AC) has recently been reported as a method to adsorb antibiotics. However, the antibiotic adsorption by AC is nonspecific and may also result in the adsorption of essential biological molecules. In this work, we reported that an anion exchange resin (AER) has better specificity than AC for adsorbing the beta-lactam antibiotic cefoperazone (CEF). Because CEF has a negatively charged carboxylate group and a conjugated system, the AER was used to adsorb CEF through electrostatic and pi-pi interactions. The AER was specific for CEF over biological molecules such as bile acids and vitamins in the intestine. The AER protected Escherichia coli from CEF in vitro. Furthermore, oral administration of the AER reduced the fecal free CEF concentration, and protected the gut microbiota from CEF-induced dysbiosis.