A permeable cuticle in Arabidopsis leads to a strong resistance to Botrytis cinerea
A permeable cuticle in Arabidopsis leads to a strong resistance to Botrytis cinerea
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DOI：
10.1038/sj.emboj.7601658
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发表时间：
2007-04-18
期刊：
影响因子：
11.4
通讯作者：
Nawrath, Christiane
中科院分区：
文献类型：
--
作者：
Bessire, Michael;Chassot, Celine;Nawrath, Christiane
由角质、脂质衍生的聚酯和角质层蜡组成的植物角质层覆盖植物的地上部分，并构成保护植物免受环境胁迫的疏水性细胞外基质层。拟南芥的葡萄孢属抗性1（bre 1）突变体揭示了可渗透的角质层一般不促进真菌病原体的进入，但令人惊讶地导致葡萄孢属的入侵被阻止。BRE 1被鉴定为长链酰基辅酶A合成酶2（LACS 2），其先前已被证明参与角质层发育并且在此被发现对于角质生物合成是必需的。bre 1/lacs 2具有五倍的二羧酸减少，二羧酸是拟南芥角质的典型单体。bre 1/lacs 2与突变体lacerata和hothead的比较显示，角质层的渗透性增加有利于感知马铃薯葡萄糖肉汤中推定的激发子，导致拟南芥植物表面存在抗真菌化合物，赋予对葡萄孢属和核盘菌属的抗性。因此，具有可渗透角质层的拟南芥植物对其环境的感知发生了改变，并相应地改变了其生理学。
The plant cuticle composed of cutin, a lipid-derived polyester, and cuticular waxes covers the aerial portions of plants and constitutes a hydrophobic extracellular matrix layer that protects plants against environmental stresses. The botrytis-resistant 1 (bre1) mutant of Arabidopsis reveals that a permeable cuticle does not facilitate the entry of fungal pathogens in general, but surprisingly causes an arrest of invasion by Botrytis. BRE1 was identified to be long-chain acyl-CoA synthetase2 (LACS2) that has previously been shown to be involved in cuticle development and was here found to be essential for cutin biosynthesis. bre1/lacs2 has a five-fold reduction in dicarboxylic acids, the typical monomers of Arabidopsis cutin. Comparison of bre1/lacs2 with the mutants lacerata and hothead revealed that an increased permeability of the cuticle facilitates perception of putative elicitors in potato dextrose broth, leading to the presence of antifungal compound(s) at the surface of Arabidopsis plants that confer resistance to Botrytis and Sclerotinia. Arabidopsis plants with a permeable cuticle have thus an altered perception of their environment and change their physiology accordingly.