Gastrointestinal synthetic epithelial linings.
Gastrointestinal synthetic epithelial linings.
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DOI：
10.1126/scitranslmed.abc0441
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发表时间：
2020-08-26
影响因子：
17.1
通讯作者：
Traverso G
中科院分区：
文献类型：
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作者：
Li J;Wang T;Kirtane AR;Shi Y;Jones A;Moussa Z;Lopes A;Collins J;Tamang SM;Hess K;Shakur R;Karandikar P;Lee JS;Huang HW;Hayward A;Traverso G
上皮组织排列在身体的器官上，提供最初的保护屏障以及营养和药物吸收的表面。在这里，我们确定了存在于胃肠道上皮中的酶成分，可以作为组织定向聚合的选择性手段。我们专注于小肠，鉴于其在药物和营养吸收中的作用，并将过氧化氢酶确定为具有催化合成生物材料层聚合和生长潜力的必需酶。我们证明，多巴胺的聚合过氧化氢酶产生强大的组织粘附。我们表征了猪和人离体胃肠道段中聚合的机制和特异性。此外，我们还证明了这些胃肠道合成上皮衬里（GSELs）应用于药物递送、酶固定用于消化补充以及通过猪体内短暂屏障形成进行营养调节的概念验证。这种基于过氧化氢酶的原位生物材料生成方法可能具有广泛的胃肠道应用适应症。
Epithelial tissues line the organs of the body, providing an initial protective barrier as well as a surface for nutrient and drug absorption. Here we identified enzymatic components present in the gastrointestinal epithelium that can serve as selective means for tissue-directed polymerization. We focused on the small intestine, given its role in drug and nutrient absorption, and identified catalase as an essential enzyme with the potential to catalyze polymerization and growth of synthetic biomaterial layers. We demonstrated that the polymerization of dopamine by catalase yields strong tissue adhesion. We characterized the mechanism and specificity of the polymerization in segments of the gastrointestinal tracts of pigs and humans ex vivo. Moreover, we demonstrated proof-of-concept for application of these gastrointestinal synthetic epithelial linings (GSELs) for drug delivery, enzymatic immobilization for digestive supplementation, and nutritional modulation through transient barrier formation in pigs. This catalase-based approach to in situ biomaterial generation may have broad indications for gastrointestinal applications.
影响因子：
16.2
作者：
通讯作者：
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