Dynamic regulation of Pep-induced immunity through post-translational control of defence transcript splicing
Dynamic regulation of Pep-induced immunity through post-translational control of defence transcript splicing
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DOI：
10.1038/s41477-020-0724-1
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发表时间：
2020-07-20
期刊：
影响因子：
18
通讯作者：
Huffaker, Alisa
中科院分区：
文献类型：
--
作者：
Dressano, Keini;Weckwerth, Philipp R.;Huffaker, Alisa
所有生物的生存都需要有探测攻击的能力，并通过保护性免疫反应迅速予以反击。尽管有相当大的机制进步，但信号模块的互连性通常仍不清楚。一种新发现的蛋白质，免疫调节rna结合蛋白（IRR），负调控玉米和拟南芥的免疫应答，功能破坏导致抗病性增强。IRR与防御信号蛋白编码转录本的典型剪接相关，包括模式识别受体信号复合物的关键负调节因子钙依赖性蛋白激酶28 （CPK28）。在植物激发肽（Plant Elicitor Peptides, pep）的免疫激活中，IRR被去磷酸化，破坏了与CPK28转录物的相互作用，导致替代剪接变异体的积累，该变异体编码截断的CPK28蛋白，其激酶活性受损，作为负调节因子的功能减弱。我们展示了一种新的机制，将Pep诱导的IRR翻译后修饰与转录后介导的CPK28功能衰减联系起来，从而动态地放大Pep信号和免疫输出。在拟南芥和玉米中，一种新发现的rna结合蛋白在免疫诱导Pep1感知后被去磷酸化。这种修饰诱导了选择性剪接介导的截断CPK28激酶的产生，其活性降低，从而增加了PEPR信号传导能力。
The survival of all living organisms requires the ability to detect attacks and swiftly counter them with protective immune responses. Despite considerable mechanistic advances, the interconnectivity of signalling modules often remains unclear. A newly characterized protein, IMMUNOREGULATORY RNA-BINDING PROTEIN (IRR), negatively regulates immune responses in both maize andArabidopsis, with disrupted function resulting in enhanced disease resistance. IRR associates with and promotes canonical splicing of transcripts encoding defence signalling proteins, including the key negative regulator of pattern-recognition receptor signalling complexes, CALCIUM-DEPENDENT PROTEIN KINASE 28 (CPK28). On immune activation by Plant Elicitor Peptides (Peps), IRR is dephosphorylated, disrupting interaction withCPK28transcripts and resulting in the accumulation of an alternative splice variant encoding a truncated CPK28 protein with impaired kinase activity and diminished function as a negative regulator. We demonstrate a new mechanism linking Pep-induced post-translational modification of IRR with post-transcriptionally mediated attenuation of CPK28 function to dynamically amplify Pep signalling and immune output.A newly identified RNA-binding protein is dephosphorylated after immunity-induced Pep1 perception inArabidopsisand maize. This modification induces alternative splicing-mediated production of a truncated CPK28 kinase with reduced activity, which increases PEPR signalling capacity.