Genetic manipulation of sex ratio for the large-scale production of all-male tilapia, Oreochromis niloticus
Genetic manipulation of sex ratio for the large-scale production of all-male tilapia, Oreochromis niloticus
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DOI：
10.1139/cjfas-54-2-396
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发表时间：
1997-02-01
影响因子：
2.4
通讯作者：
Beardmore, JA
中科院分区：
文献类型：
--
作者：
Mair, GC;Abucay, JS;Beardmore, JA
我们描述了尼罗罗非鱼，Oreochromis niloticus，纳入雌激素诱导的性反转的男性女性和后代测试，以产生新的“YY”男性基因型的育种计划的结果。“YY”雄性基因型被证明是可行的和肥沃的正常XY男性和父系的平均后代性别比为95.6%的男性。虽然这些结果证实了主要是单因素性别决定的假设，似乎有一个遗传基础的发生偶尔女性的后代中的“YY”男性。这些很可能是通过几个常染色体性别修饰基因的作用而产生的。我们报告的第一个实例的雌性化的YY基因型罗非鱼和大规模生产的“YY”男性通过YY × YY交配，避免了耗时的后代测试来区分XY和YY男性基因型。这使得“YY”雄性和它们的全雄性后代（称为遗传雄性罗非鱼）的生产能够以商业规模大规模生产。我们的结论是，YY雄性技术提供了一个强大的和可靠的解决方案，严重和普遍的问题，性成熟过早，不必要的繁殖，并在罗非鱼养殖过剩。
We describe the results of a breeding programme for Nile tilapia, Oreochromis niloticus, incorporating estrogen-induced sex reversal of male to female and progeny testing to generate novel 'YY' male genotypes. 'YY' male genotypes proved to be as viable and fertile as normal XY males and to sire a mean progeny sex ratio of 95.6% male. While these results confirm the hypothesis of predominantly monofactorial sex determination, there appears to be a genetic basis for the occurrence of occasional females in the progeny of 'YY' males. It is likely that these arise through the action of several autosomal sex modifying genes. We report the first instance of feminization of YY genotypes in tilapia and the mass production of 'YY' males through YY x YY matings, obviating the need for time-consuming progeny testing to discriminate XY and YY male genotypes. This enables the production of 'YY' males and their all-male progeny, known as genetically male tilapia, to be mass produced on a commercial scale. We conclude that the YY male technology provides a robust and reliable solution to the serious and widespread problem of early sexual maturation, unwanted reproduction, and overpopulation in tilapia culture.