Development of a V-shape bis(tetraoxacalix[2]arene[2]triazine) stationary phase for High performance liquid chromatography
Development of a V-shape bis(tetraoxacalix[2]arene[2]triazine) stationary phase for High performance liquid chromatography
复制标题
高效液相色谱V型双(四氧萼[2]芳烃[2]三嗪)固定相的研制
DOI：
10.1016/j.talanta.2014.06.054
复制
发表时间：
2014-12-01
期刊：
影响因子：
6.1
通讯作者：
Zhang, Shusheng
中科院分区：
文献类型：
--
作者：
Hu, Kai;Feng, Suxiang;Zhang, Shusheng
以3-氨丙基三乙氧基硅烷为偶联剂，将V型笼状杂原子桥联杯芳烃共价键合到硅胶上，制备了一种新型高效液相色谱固定相。采用红外光谱、元素分析和热重分析对材料的结构进行了表征。采用线性溶剂化能关系法对34种溶质进行了评价。新相的保留特性与ODS有着显著的相似性，但也有明显的特点。涉及到疏水、氢键、π-π和n-pi相互作用等多种机制。以烷基苯、芳烃位置异构体和黄酮类化合物为探针，考察了该相的色谱行为。此外，无机阴离子单独分离的阴离子交换模式，通过使用相同的柱。因此，新相的多重相互作用机制和混合模式分离很可能保证其在复杂样品分析中具有优异的色谱性能。该色谱柱已成功地用于动物尿液中克伦特罗的分析，证明了该色谱柱是一种合适的和有竞争力的替代分析方法，用于测定克伦特罗。(C)2014爱思唯尔有限公司版权所有。
A new stationary phase for high-performance liquid chromatography was prepared by covalently bonding a V-shape cage heteroatom-bridged calixarene onto silica gel using 3-aminopropyltriethoxysilane as coupling reagent. The structure of the new material was characterized by infrared spectroscopy, elemental analysis and thermogravimetric analysis. Linear solvation energy relationship method was successfully employed to evaluate the new phase with a set of 34 solutes. The retention characteristic of the new phase shows significant similarities with ODS, as well as distinctive features. Multiple mechanisms including hydrophobic, hydrogen bonding, pi-pi and n-pi interaction are involved. The chromatographic behavior of the phase was illustrated by using alkylbenzenes, aromatics positional isomers and flavonoids as probes. Moreover, inorganic anions were individually separated in anion-exchange mode by using the same column. Thus, multi-interaction mechanisms and mixed-mode separation of the new phase can very likely guarantee its excellent chromatographic performance for the analysis of complex samples. The column has been successfully employed for the analysis of clenbuterol in animal urine, and it is demonstrated to be suitable and a competitive alternative analytical method for the determination of clenbuterol. (C) 2014 Elsevier B.V. All rights reserved.