Circulating Neutrophil MicroRNAs as Biomarkers for the Detection of Lung Cancer.
Circulating Neutrophil MicroRNAs as Biomarkers for the Detection of Lung Cancer.
复制标题
DOI：
10.4137/bic.s37333
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Jiang F
中科院分区：
文献类型：
--
作者：
Ma J;Li N;Lin Y;Gupta C;Jiang F
外周中性粒细胞是主要的循环白细胞，也是先天性和适应性免疫系统的重要组成部分，是抵抗癌症的主要防御手段。 MicroRNA (miRNA) 可以调节中性粒细胞功能，并通过调节中性粒细胞基因表达在癌症发病机制中发挥重要作用。为了调查外周中性粒细胞差异 miRNA 水平的评估是否有可能诊断非小细胞肺癌 (NSCLC)，我们检查了 15 名 I 期 NSCLC 患者和 15 名未患癌症的吸烟者的中性粒细胞。我们发现 NSCLC 患者与非癌症吸烟者中的 5 种中性粒细胞 miRNA 水平异常。在 82 名肺癌患者和 73 名对照患者的训练集中，开发了一组两个基因（miRs-26a-2-3p 和 574-3p），对 NSCLC 检测产生 77.8% 的敏感性和 78.1% 的特异性。此外，在 60 名肺癌患者和 58 名吸烟者的测试组中，分析这两种 miRNA 用于肺癌检测的性能得到了证实。这项研究首次表明中性粒细胞 miRNA 谱可作为一类新的循环生物标志物用于检测 NSCLC。
Peripheral neutrophils are the predominant circulating leukocytes and an important component of innate and adaptive immune systems, which is a primary defense against cancer. MicroRNAs (miRNAs) can modulate neutrophil functions and play important roles in cancer pathogenesis by regulating neutrophil gene expression. To investigate if assessment of differential miRNA levels of peripheral neutrophils has the potential for diagnosis of non-small-cell lung cancer (NSCLC), we examine neutrophils of 15 patients with stage I NSCLC and 15 smokers without cancer. We identify five neutrophil miRNAs that have an abnormal level in patients with NSCLC versus smokers without cancer. In a training set of 82 patients with lung cancer and 73 controls, a set of two genes (miRs-26a-2-3p and 574-3p) are developed, producing 77.8% sensitivity and 78.1% specificity for NSCLC detection. Furthermore, in a testing set of 60 patients with lung cancer and 58 smokers, the performance of analyzing the two miRNAs for lung cancer detection is confirmed. This study for the first time shows that a neutrophil miRNA profile may serve as a new category of circulating biomarkers for the detection of NSCLC.