Simultaneous determination of epinephrine and norepinephrine in human blood plasma and urine samples using nanotubes modified edge plane pyrolytic graphite electrode
Simultaneous determination of epinephrine and norepinephrine in human blood plasma and urine samples using nanotubes modified edge plane pyrolytic graphite electrode
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DOI：
10.1016/j.talanta.2010.12.034
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发表时间：
2011-03-15
期刊：
影响因子：
6.1
通讯作者：
Bishnoi, Sunita
中科院分区：
文献类型：
--
作者：
Goyal, Rajendra N.;Bishnoi, Sunita
采用多壁碳纳米管修饰的边缘平面热解石墨电极（MWNT/EPPGE），在生理pH值为7.2的条件下，用方波伏安法（SWV）同时测定儿茶酚胺-肾上腺素和去甲肾上腺素。在裸EPPGE中，肾上腺素（EP）和去甲肾上腺素（NE）的氧化出现了一个类似于250 mV的宽峰，而在MWNT/EPPGE中，EP和NE的氧化分别出现了两个类似于150 mV和215 mV的分离良好的峰。在MWNT/EPPGE作用下，两种神经递质氧化峰电流也随着峰电位的负移而显著增加。两种化合物的氧化均发生在pH依赖的2H和2H（+）过程中，电极反应遵循扩散控制途径。在0.5 ~ 100 nM范围内，肾上腺素和去甲肾上腺素的检出限分别为0.15 × 10(-9) m和0.90 × 10(-10) m，得到线性校准曲线。开发的方案是实施的同时测定血浆和尿样中的肾上腺素和去甲肾上腺素吸烟者以及运动员。(C) 2010 Elsevier B.V.版权所有
The simultaneous determination of catecholamines - epinephrine and norepinephrine by square wave voltammetry (SWV) at physiological pH 7.2 is reported using multi-walled carbon nanotubes modified edge plane pyrolytic graphite electrode (MWNT/EPPGE). A broad bump at similar to 250 mV is appeared for the oxidation of epinephrine (EP) and norepinephrine (NE) at bare EPPGE whereas at MWNT/EPPGE two well-separated peaks at similar to 150 and similar to 215 mV are appeared for the oxidation of EP and NE, respectively. The oxidation peak current of both the neurotransmitters also increased significantly along with the negative shift of peak potentials using MWNT/EPPGE. The oxidation of both compounds occurred in a pH dependent, 2e and 2H(+) process and the electrode reaction followed diffusion controlled pathway. Linear calibration curves were obtained for epinephrine and norepinephrine in the range 0.5-100 nM with limits of detection 0.15 x 10(-9) and 0.90 x 10(-10) M. respectively. The developed protocol is implemented for the simultaneous determination of epinephrine and norepinephrine in blood plasma and urine samples of smokers as well as in athletes. (C) 2010 Elsevier B.V. All rights reserved.